Rabbit Anti-MAD2L1 Polyclonal Antibody – Cat# MBS617331

MAD2L1 (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.

MAD2L1 Antibody – Rabbit Polyclonal

Name: MAD2L1 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS617331
Price: 785 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS617331

MBS617331 is an antibody targeting MAD2L1. Also known as MAD2L1 (Mitotic Spindle Assembly Checkpoint Protein MAD2A, MAD2-like 1, HsMAD2). Reactive in Chicken, Human, Mouse. Host: Rabbit. More details

$785 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MAD2L1, Polyclonal Antibody

Full Product Name

MAD2L1 (Mitotic Spindle Assembly Checkpoint Protein MAD2A, MAD2-like 1, HsMAD2)

Product Gene Name

anti-MAD2L1 antibody

Product Synonym Names

Anti -MAD2L1 (Mitotic Spindle Assembly Checkpoint Protein MAD2A, MAD2-like 1, HsMAD2)

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Chicken, Human, Mouse

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes Human MAD2 Mitotic Arrest Deficient-like 1 (MAD2L1). Species Crossreactivity: Chicken, Human, Mouse.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

23,510 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (120980577..120988013, complement). Location: 4q27

OMIM

601467

3D Structure

ModBase 3D Structure for Q13257

Immunogen

Synthetic peptide, KLH-conjugated. Epitope: Amino acid residues 3-13 of Human MAD2L1 protein.

Product Categories/Family for anti-MAD2L1 antibody

Antibodies; Abs to Cell Division Cycle (CDC)

Applications Tested/Suitable for anti-MAD2L1 antibody

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Application Notes for anti-MAD2L1 antibody

Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry: 2.5ug/ml
Western Blot: 1:500-1:2000
ELISA: 1:2000-1:10,000

Other Notes

Small volumes of anti-MAD2L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAD2L1. It may not necessarily be applicable to this product.

NCBI GI #

4505067

NCBI GeneID

4085

NCBI Accession #

NP_002349.1

UniProt Primary Accession #

Q13257

UniProt Secondary Accession #

Q53F56; Q548X9; Q6IRW7; Q8IZX3

UniProt Related Accession #

Q13257

GenBank Nucleotide #

NM_002358.3

NCBI Official Full Name

mitotic spindle assembly checkpoint protein MAD2A

NCBI Official Synonym Full Names

MAD2 mitotic arrest deficient-like 1 (yeast)

NCBI Official Symbol

MAD2L1

NCBI Official Synonym Symbols

MAD2; HSMAD2

UniProt Protein Name

Mitotic spindle assembly checkpoint protein MAD2A

UniProt Synonym Protein Names

Mitotic arrest deficient 2-like protein 1

Protein Family

Mitotic spindle assembly checkpoint protein

UniProt Gene Name

MAD2L1

UniProt Synonym Gene Names

MAD2; HsMAD2

UniProt Entry Name

MD2L1_HUMAN

NCBI Protein Information

mitotic spindle assembly checkpoint protein MAD2A; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1

NCBI Summary for MAD2L1

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]

UniProt Comments for MAD2L1

MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: kinetochore; spindle pole; perinuclear region of cytoplasm; nuclear pore; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of mitotic cell cycle; mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of apoptosis

Research Articles on MAD2L1

1. MAD2 expression levels can indicate sensitivity to anticancer agents, and risk for recurrence in ovarian serous adenocarcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAD2L1 antibody

Reference Product	PubMed Publications
CDC20 antibody	>85 publications with MAD2L1 and CDC20
MAD1L1 antibody	>46 publications with MAD2L1 and MAD1L1
BUB1B antibody	>14 publications with MAD2L1 and BUB1B
TTK antibody	>13 publications with MAD2L1 and TTK
MAD2L1BP antibody	>13 publications with MAD2L1 and MAD2L1BP
NDC80 antibody	>7 publications with MAD2L1 and NDC80
CDC16 antibody	>1 publications with MAD2L1 and CDC16
CDC27 antibody	>1 publications with MAD2L1 and CDC27

Pathways associated with anti-MAD2L1 antibody

Products by Pathway	Pathway Diagram
APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies	APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway antibodies	Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway Diagram
Amplification Of Signal From The Kinetochores Pathway antibodies	Amplification Of Signal From The Kinetochores Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram

Diseases associated with anti-MAD2L1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>112 publications with MAD2L1 and Neoplasms
Carcinoma Antibodies	>52 publications with MAD2L1 and Carcinoma
Adenocarcinoma Antibodies	>27 publications with MAD2L1 and Adenocarcinoma
Breast Neoplasms Antibodies	>17 publications with MAD2L1 and Breast Neoplasms
Cell Transformation, Neoplastic Antibodies	>13 publications with MAD2L1 and Cell Transformation, Neoplastic
Liver Diseases Antibodies	>10 publications with MAD2L1 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>10 publications with MAD2L1 and Carcinoma, Hepatocellular
Ovarian Neoplasms Antibodies	>9 publications with MAD2L1 and Ovarian Neoplasms
Neoplasm Metastasis Antibodies	>8 publications with MAD2L1 and Neoplasm Metastasis
Liver Neoplasms Antibodies	>8 publications with MAD2L1 and Liver Neoplasms

Organs/Tissues associated with anti-MAD2L1 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>12 publications with MAD2L1 and Lung
Stomach Antibodies	>10 publications with MAD2L1 and Stomach
Liver Antibodies	>10 publications with MAD2L1 and Liver
Prostate Antibodies	>7 publications with MAD2L1 and Prostate
Blood Antibodies	>7 publications with MAD2L1 and Blood
Bone Antibodies	>6 publications with MAD2L1 and Bone
Lymph Antibodies	>5 publications with MAD2L1 and Lymph
Skin Antibodies	>4 publications with MAD2L1 and Skin
Brain Antibodies	>4 publications with MAD2L1 and Brain
Kidney Antibodies	>3 publications with MAD2L1 and Kidney