• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

RIOK3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6175039

This gene was identified by the similarity of its product to the Aspergillus nidulans SUDD protein, an extragenic suppressor of the heat-sensitive bimD6 mutation that fails to attach properly to the spindle microtubules at a restrictive temperature. The… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RIOK3, Monoclonal Antibody
Full Product Name
RIOK3 (RIO Kinase 3 (yeast), DKFZp779L1370, SUDD) (FITC)
Product Gene Name
anti-RIOK3 antibody
Product Synonym Names
RIO Kinase 3 (yeast); DKFZp779L1370; SUDD
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4A12-1D2
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified
Specificity
Recognizes RIOK3.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RIOK3 (AAH39729, 1aa-516aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Conjugate
FITC

Product Categories/Family for anti-RIOK3 antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-RIOK3 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-RIOK3 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RIOK3 antibody

RIOK3 monoclonal antibody (M01), clone 4A12-1D2 Western Blot analysis of RIOK3 expression in Hela S3 NE (Cat # L013V3).
Mouse Anti-RIOK3 Monoclonal Antibody – Cat# MBS6175039

Testing Data of anti-RIOK3 antibody

Detection limit for recombinant GST tagged RIOK3 is approximately 0.3ng/ml as a capture antibody.
Mouse Anti-RIOK3 Monoclonal Antibody, Testing Data – Cat# MBS6175039

Immunoprecipitation (IP) of anti-RIOK3 antibody

Immunoprecipitation of RIOK3 transfected lysate using anti-RIOK3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RIOK3 MaxPab rabbit polyclonal antibody.
Mouse Anti-RIOK3 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6175039

Western Blot (WB) of anti-RIOK3 antibody

Western Blot analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M01), clone 4A12-1D2.

Lane 1: RIOK3 transfected lysate (59.1 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-RIOK3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6175039

Product References and Citations for anti-RIOK3 antibody

1. Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer. Kimmelman AC, Hezel AF, Aguirre AJ, Zheng H, Paik JH, Ying H, Chu GC, Zhang JX, Sahin E, Yeo G, Ponugoti A, Nabioullin R, Deroo S, Yang S, Wang X, McGrath JP, Protopopova M, Ivanova E, Zhang J, Feng B, Tsao MS, Redston M, Protopopov A, Xiao Y, Futreal PA,Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19372-7. Epub 2008 Dec 2.

Other Notes

Small volumes of anti-RIOK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RIOK3 purchase

MBS6175039 is an antibody targeting RIOK3. Also known as RIOK3 (RIO Kinase 3 (yeast), DKFZp779L1370, SUDD) (FITC). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RIOK3. It may not necessarily be applicable to this product.

NCBI GI #
24981029
NCBI GeneID
8780
NCBI Accession #
AAH39729
UniProt Related Accession #
O14730
NCBI Official Full Name
RIOK3 protein
NCBI Official Synonym Full Names
RIO kinase 3
NCBI Official Symbol
RIOK3
NCBI Official Synonym Symbols
SUDD
NCBI Protein Information
serine/threonine-protein kinase RIO3

NCBI Summary for RIOK3

This gene was first identified by the similarity of its product to the Aspergillus nidulans SUDD protein. This gene is now recognized as a member of the right open reading frame (RIO) kinase gene family. This gene encodes a serine/threonine kinase that localizes to the cytoplasm and plays a role in the processing of the pre-40 S ribosomal subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2017]
Show more

Research Articles on RIOK3

1. RIO kinase 3 (RIOK3) positively regulates the activity of the AKT/mTOR pathway in glioma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000