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UBA1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6178789

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
UBA1, Monoclonal Antibody
Full Product Name
UBA1 (Ubiquitin-like modifier Activating Enzyme 1, A1S9, A1S9T, A1ST, AMCX1, GXP1, MGC4781, SMAX2, UBA1A, UBE1, UBE1X) (FITC)
Product Gene Name
anti-UBA1 antibody
Product Synonym Names
Ubiquitin-like modifier Activating Enzyme 1; A1S9; A1S9T; A1ST; AMCX1; GXP1; MGC4781; SMAX2; UBA1A; UBE1; UBE1X
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2B6
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified
Specificity
Recognizes UBA1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
OMIM
phenotype 314370
3D Structure
ModBase 3D Structure for P22314
Immunogen
UBA1 (NP_003325.2, 1aa-1058aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Conjugate
FITC

Product Categories/Family for anti-UBA1 antibody

Antibodies; Ubiquitin
Applications Tested/Suitable for anti-UBA1 antibody
Western Blot (WB)
Application Notes for anti-UBA1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-UBA1 antibody

UBA1 monoclonal antibody (M02), clone 2B6. Western Blot analysis of UBA1 expression in HeLa.
Mouse Anti-UBA1 Monoclonal Antibody – Cat# MBS6178789

Testing Data of anti-UBA1 antibody

Detection limit for recombinant GST tagged UBA1 is 0.3 ng/ml as a capture antibody.
Mouse Anti-UBA1 Monoclonal Antibody, Testing Data – Cat# MBS6178789

Other Notes

Small volumes of anti-UBA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBA1 purchase

MBS6178789 is an antibody targeting UBA1. Also known as UBA1 (Ubiquitin-like modifier Activating Enzyme 1, A1S9, A1S9T, A1ST, AMCX1, GXP1, MGC4781, SMAX2, UBA1A, UBE1, UBE1X) (FITC). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for UBA1. It may not necessarily be applicable to this product.

NCBI GI #
23510338
NCBI GeneID
7317
NCBI Accession #
NP_003325.2
UniProt Primary Accession #
P22314
UniProt Secondary Accession #
Q5JRR8; Q96E13
UniProt Related Accession #
P22314
GenBank Nucleotide #
NM_003334.3
NCBI Official Full Name
ubiquitin-like modifier-activating enzyme 1
NCBI Official Synonym Full Names
ubiquitin like modifier activating enzyme 1
NCBI Official Symbol
UBA1
NCBI Official Synonym Symbols
A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124
UniProt Protein Name
Ubiquitin-like modifier-activating enzyme 1
UniProt Synonym Protein Names
Protein A1S9; Ubiquitin-activating enzyme E1
Protein Family
Ubiquitin-activating enzyme
UniProt Gene Name
UBA1
UniProt Synonym Gene Names
A1S9T; UBE1
UniProt Entry Name
UBA1_HUMAN
NCBI Protein Information
ubiquitin-like modifier-activating enzyme 1

UniProt Comments for UBA1

UBE1: an enzyme of the ubiquitin-activating E1 family. Adenylates ubiquitin at its carboxy-terminal glycine residue and thereafter links this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester. There are two active sites within the E1 molecule, allowing it to accomodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: heterochromatin; desmosome; mitochondrion; lysosomal membrane; cytoplasm; endosome membrane; rough endoplasmic reticulum membrane; nucleus; cytosol

Molecular Function: protein binding; ubiquitin-protein ligase activity; ubiquitin activating enzyme activity; ATP binding

Biological Process: modification-dependent protein catabolic process; protein ubiquitination; response to DNA damage stimulus

Disease: Spinal Muscular Atrophy, X-linked 2
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Research Articles on UBA1

1. A ubiquitin shuttle DC-UbP reconciles protein ubiquitination and deubiquitination via linking UbE1 and USP5 enzymes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBA1 antibody

 Reference Product  PubMed Publications
 UBC antibody  >2 publications with UBA1 and UBC
 UBE2A antibody  >1 publications with UBA1 and UBE2A

Pathways associated with anti-UBA1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Parkin-Ubiquitin Proteasomal System Pathway antibodies  Parkin-Ubiquitin Proteasomal System Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Parkinsons Disease Pathway antibodies  Parkinsons Disease Pathway Diagram
 Proteasome Degradation Pathway antibodies  Proteasome Degradation Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-UBA1 antibody

 Disease Name  Pubmed Publications
 Atrophy Antibodies  >10 publications with UBA1 and Atrophy
 Death Antibodies  >9 publications with UBA1 and Death
 Nervous System Diseases Antibodies  >8 publications with UBA1 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >3 publications with UBA1 and Cardiovascular Diseases
 Weight Loss Antibodies  >3 publications with UBA1 and Weight Loss
 Brain Diseases Antibodies  >3 publications with UBA1 and Brain Diseases
 Necrosis Antibodies  >3 publications with UBA1 and Necrosis
 Inflammation Antibodies  >2 publications with UBA1 and Inflammation
 Adenocarcinoma Antibodies  >2 publications with UBA1 and Adenocarcinoma
 Heart Diseases Antibodies  >2 publications with UBA1 and Heart Diseases

Organs/Tissues associated with anti-UBA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >7 publications with UBA1 and Muscle
 Brain Antibodies  >7 publications with UBA1 and Brain
 Nerve Antibodies  >4 publications with UBA1 and Nerve
 Eye Antibodies  >3 publications with UBA1 and Eye
 Spinal Cord Antibodies  >3 publications with UBA1 and Spinal Cord
 Blood Antibodies  >3 publications with UBA1 and Blood
 Intestine Antibodies  >2 publications with UBA1 and Intestine
 Lung Antibodies  >2 publications with UBA1 and Lung
 Embryonic Tissue Antibodies  >2 publications with UBA1 and Embryonic Tissue
 Mouth Antibodies  >2 publications with UBA1 and Mouth
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