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Napi-4, Nt, Aa71-86 Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS618078

MBS618078 is an antibody targeting NaPi-4, NT, aa71-86. Also known as Anti - NaPi-4, NT, aa71-86 (Npt2a). Host: ChickenSource: Opossum. More details

$965 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NaPi-4, NT, aa71-86, Polyclonal Antibody
Full Product Name
Anti - NaPi-4, NT, aa71-86 (Npt2a)
Product Synonym Names
NaPi-4, NT, aa71-86 (Npt2a)
Clonality
Polyclonal
Isotype
IgY
Host
Host: Chicken
Source: Opossum
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Purity/Purification
Affinity purified over the control-peptide Sepharose
Specificity
Peptide is specific for opossum Napi-4/npt2a. This sequence is not conserved in mouse, rat, human and other species Npt2a. No significant sequence homology is observed with other Pi-transporters (Type-I, III, or IV) transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 20 AA synthetic peptide within the N-terminus region (71-86 aa) of Opossum NaPi-4/NPT2a was synthesized, conjugated to KLH

Product Categories/Family for anti-NaPi-4, NT, aa71-86 antibody

Abs to Ion Transport
Applications Tested/Suitable for anti-NaPi-4, NT, aa71-86 antibody
ELISA, Western Blot
Application Notes for anti-NaPi-4, NT, aa71-86 antibody
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Product References and Citations for anti-NaPi-4, NT, aa71-86 antibody

• 1). Sorribas V et al (1994) JBC 269, 6615-6621; Magnin S et al (1993) PNAS 90, 5979; Collins JF et al (1996) FASEB J 8, 862; Hartman CM et al (1996) PNAS 93, 7409.
• 2. Murer H et al (2000) Physiol. Rev. 80, 1373 (review); Werner A et al (2001) Am. J. Physiol. Integ. Comp. Physiol. 280, R301 (review).

Other Notes

Small volumes of anti-NaPi-4, NT, aa71-86 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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