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Proteasome 19s, Subunit S5A (RPN10) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS618137

S5a(PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinylated-protein targeting and degradation. S5a contains two polyubiquitin binding motifs (UIM) which bind multiubiquitin chains by hydrophobic interaction. In addition to… More details

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Product Overview

Online Datasheet in PDF
Product Name
Proteasome 19S, Subunit S5A (RPN10), Polyclonal Antibody
Full Product Name
Proteasome 19S, Subunit S5A (PSMD4)
Product Gene Name
Product Synonym Names
Anti -Proteasome 19S, Subunit S5A (PSMD4)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Specificity
Detects endogenous levels of total human S5a/PSMD4 protein, 49kD. Species Crossreactivity: Monkey, mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
49kD
Chromosome Location
Chromosome: VIII; NC_001140.6 (499079..499885). Location: chromosome: VIII
3D Structure
ModBase 3D Structure for P38886
Immunogen
Synthetic peptide corresponding to residues surrounding Gly344 of human S5a/PSMD4 protein (KLH).

Product Categories/Family for anti-RPN10 antibody

Applications Tested/Suitable for anti-RPN10 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-RPN10 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000

Western Blot (WB) of anti-RPN10 antibody

Western blot analysis of extracts from various cell types using MBS618137.
Rabbit Anti-RPN10 Polyclonal Antibody – Cat# MBS618137

Other Notes

Small volumes of anti-RPN10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPN10 purchase

MBS618137 is an antibody targeting RPN10. Also known as Proteasome 19S, Subunit S5A (PSMD4). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPN10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
proteasome regulatory particle base subunit RPN10
NCBI Official Symbol
RPN10
NCBI Official Synonym Symbols
MCB1; SUN1
UniProt Protein Name
26S proteasome regulatory subunit RPN10
UniProt Gene Name
RPN10
UniProt Synonym Gene Names
MCB1; SUN1
UniProt Entry Name
RPN10_YEAST
NCBI Protein Information
proteasome regulatory particle base subunit RPN10

UniProt Comments for RPN10

Function: Multiubiquitin binding protein.

Subunit structure: The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively

By similarity.

Miscellaneous: Present with 17200 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the proteasome subunit S5A family.Contains 1 UIM (ubiquitin-interacting motif) repeat.Contains 1 VWFA domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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