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ACTB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6181590

MBS6181590 is an antibody targeting ACTB. Also known as ACTB (Actin, beta, PS1TP5BP1) (HRP). Reactive in Human, Mouse, Rat. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ACTB, Monoclonal Antibody
Full Product Name
ACTB (Actin, beta, PS1TP5BP1) (HRP)
Product Gene Name
Product Synonym Names
Actin; beta; PS1TP5BP1
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
10000000
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified
Specificity
Recognizes Human, Mouse, Rat ACTB.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ACTB (AAH01301, 1aa-375aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVSPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERRYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Conjugate
HRP
Applications Tested/Suitable for anti-ACTB antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ACTB antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ACTB antibody

ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Jurkat (Cat # L017V1).
Mouse Anti-ACTB Monoclonal Antibody – Cat# MBS6181590

Immunohistochemistry (IHC) of anti-ACTB antibody

Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
Mouse Anti-ACTB Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6181590

Immunohistochemistry (IHC) of anti-ACTB antibody

Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
Mouse Anti-ACTB Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6181590

Western Blot (WB) of anti-ACTB antibody

ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in NIH/3T3.
Mouse Anti-ACTB Monoclonal Antibody, Western Blot (WB) – Cat# MBS6181590

Western Blot (WB) of anti-ACTB antibody

ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in PC-12.
Mouse Anti-ACTB Monoclonal Antibody, Western Blot (WB) – Cat# MBS6181590

Western Blot (WB) of anti-ACTB antibody

ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.
Mouse Anti-ACTB Monoclonal Antibody, Western Blot (WB) – Cat# MBS6181590

Other Notes

Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
Actin, beta
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
Protein Family
NCBI Protein Information
actin, cytoplasmic 1

NCBI Summary for ACTB

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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