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C-Phospholipase A2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS618221

Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and the release of arachidonic acid. cPLA2 has been implicated in diverse cellular… More details

$760 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
c-Phospholipase A2, Polyclonal Antibody
Full Product Name
c-Phospholipase A2 (Cytosolic PLA2, cPLA2)
Product Synonym Names
Anti -c-Phospholipase A2 (Cytosolic PLA2, cPLA2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes endogenous levels of total human cPLA2. Does not crossreact with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2). Species crossreactivity: monkey, mouse, and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the C-terminal residues of human c-Phospholipase A2.
Grade
Affinity Purified
Applications Tested/Suitable for anti-c-Phospholipase A2 antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-c-Phospholipase A2 antibody
Suitable for use in ELISA, Western Blot and Immunoprecipitation.
WB: 1:1000
Incubate membrane with diluted MBS618221 in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight .
Immunoprecipitation (IP): 1:50
Optimal dilutions to be determined by the end researcher.
Other applications have not been tested.

Western Blot (WB) of anti-c-Phospholipase A2 antibody

Immunoprecipitation of cPLA2 from untreated and TNF-alpha treated HeLa cells followed by Western blot analysis, using MBS618221.
Rabbit Anti-C-Phospholipase A2 Polyclonal Antibody – Cat# MBS618221

Western Blot (WB) of anti-c-Phospholipase A2 antibody

Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, using MBS618221.
Rabbit Anti-C-Phospholipase A2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS618221

Other Notes

Small volumes of anti-c-Phospholipase A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for c-Phospholipase A2 purchase

MBS618221 is an antibody targeting c-Phospholipase A2. Also known as c-Phospholipase A2 (Cytosolic PLA2, cPLA2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for c-Phospholipase A2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
Chain C, Phospholipase A2 (Pla2) From Naja Naja Venom

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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