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MAPK3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6182220

MBS6182220 is an antibody targeting MAPK3. Also known as MAPK3 (Mitogen-Activated Protein Kinase 3, ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3) (HRP). Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAPK3, Monoclonal Antibody
Full Product Name
MAPK3 (Mitogen-Activated Protein Kinase 3, ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3) (HRP)
Product Gene Name
Product Synonym Names
Mitogen-Activated Protein Kinase 3; ERK1; HS44KDAP; HUMKER1A; MGC20180; P44ERK1; P44MAPK; PRKM3
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3D6
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified
Specificity
Recognizes MAPK3.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MAPK3 (AAH13992, 279aa-379aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
NYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP
Conjugate
HRP

Product Categories/Family for anti-MAPK3 antibody

Applications Tested/Suitable for anti-MAPK3 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-MAPK3 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MAPK3 antibody

MAPK3 monoclonal antibody (M05), clone 3D6. Western Blot analysis of MAPK3 expression in Hela S3 NE (Cat # L013V3).
Mouse Anti-MAPK3 Monoclonal Antibody – Cat# MBS6182220

Western Blot (WB) of anti-MAPK3 antibody

MAPK3 monoclonal antibody (M05), clone 3D6. Western Blot analysis of MAPK3 expression in NIH/3T3.
Mouse Anti-MAPK3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6182220

Western Blot (WB) of anti-MAPK3 antibody

MAPK3 monoclonal antibody (M05), clone 3D6. Western Blot analysis of MAPK3 expression in PC-12.
Mouse Anti-MAPK3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6182220

Western Blot (WB) of anti-MAPK3 antibody

MAPK3 monoclonal antibody (M05), clone 3D6. Western Blot analysis of MAPK3 expression in Raw 264.7.
Mouse Anti-MAPK3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6182220

Western Blot (WB) of anti-MAPK3 antibody

Western Blot analysis of MAPK3 expression in transfected 293T cell line by MAPK3 monoclonal antibody (M05), clone 3D6.

Lane 1: MAPK3 transfected lysate (43.1 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-MAPK3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6182220

Immunofluorescence (IF) of anti-MAPK3 antibody

Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-MAPK3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6182220

Other Notes

Small volumes of anti-MAPK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAPK3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
Mitogen-activated protein kinase 3
NCBI Official Synonym Full Names
mitogen-activated protein kinase 3
NCBI Official Symbol
MAPK3
NCBI Official Synonym Symbols
ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
NCBI Protein Information
mitogen-activated protein kinase 3

NCBI Summary for MAPK3

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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