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POLR2F Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6183143

MBS6183143 is an antibody targeting POLR2F. Also known as POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F, HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6) (HRP). Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLR2F, Monoclonal Antibody
Full Product Name
POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F, HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6) (HRP)
Product Gene Name
Product Synonym Names
Polymerase (RNA) II (DNA Directed) Polypeptide F; HRBP14.4; POLRF; RPABC2; RPB14.4; RPB6
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2G2
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified
Specificity
Recognizes POLR2F.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
14,478 Da
Immunogen
POLR2F (NP_068809.1, 1aa-127aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIITD
Conjugate
HRP

Product Categories/Family for anti-POLR2F antibody

Applications Tested/Suitable for anti-POLR2F antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-POLR2F antibody
Applications are based on unconjugated antibody.

Testing Data of anti-POLR2F antibody

Detection limit for recombinant GST tagged POLR2F is 0.3 ng/ml as a capture antibody.
Mouse Anti-POLR2F Monoclonal Antibody – Cat# MBS6183143

Western Blot (WB) of anti-POLR2F antibody

Western Blot analysis of POLR2F expression in transfected 293T cell line by POLR2F monoclonal antibody (M02), clone 2G2.

Lane 1: POLR2F transfected lysate (Predicted MW: 14.5 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-POLR2F Monoclonal Antibody, Western Blot (WB) – Cat# MBS6183143

Immunofluorescence (IF) of anti-POLR2F antibody

Immunofluorescence of monoclonal antibody to POLR2F on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-POLR2F Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6183143

Immunofluorescence (IF) of anti-POLR2F antibody

Immunofluorescence of monoclonal antibody to POLR2F on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-POLR2F Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6183143

Other Notes

Small volumes of anti-POLR2F antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR2F. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), transcript variant 1, mRNA
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide F
NCBI Official Symbol
POLR2F
NCBI Official Synonym Symbols
RPB6; POLRF; RPC15; RPABC2; RPB14.4; HRBP14.4; RPABC14.4
NCBI Protein Information
DNA-directed RNA polymerases I, II, and III subunit RPABC2; DNA-directed RNA polymerase II subunit F; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; RNA Polymerase II subunit 14.4 kD; RNA polymerases I, II, and III subunit ABC2

NCBI Summary for POLR2F

This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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