Rabbit Anti-PLCB3 Polyclonal Antibody – Cat# MBS618384

Western blot analysis of extracts from HeLa cells, untreated or PMA-treated for the indicated times, using Phospho-PLCbeta3 (Ser1105) MBS618384 (upper) or PLCbeta3 P4074-40G (lower).

Phospholipase C, Beta 3 (PLCB3) Antibody – Rabbit Polyclonal

Name: PLCB3 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS618384
Price: 830 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS618384

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to… More details

$830 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Phospholipase C, beta 3 (PLCB3), Polyclonal Antibody

Full Product Name

Phospholipase C, beta 3, phosphorylated (Ser1105) (PLCb3)

Product Gene Name

anti-PLCB3 antibody

Product Synonym Names

Anti -Phospholipase C, beta 3, phosphorylated (Ser1105) (PLCb3)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Monkey, Mouse, Rat

Form/Format

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human PLCbeta3 when phosphorylated at Ser1105. Does not crossreact with phosphorylated PLCbeta1, PLCbeta2, PLCbeta4 or other PLCs. Species Crossreactivity: Human, rat and monkey.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

138,799 Da

Chromosome Location

Chromosome: 11; NC_000011.9 (64018995..64036924). Location: 11q13

OMIM

600230

3D Structure

ModBase 3D Structure for Q01970

Immunogen

Synthetic phosphopeptide surrounding Ser1105 of human PLCbeta3 (KLH coupled).

Product Categories/Family for anti-PLCB3 antibody

Antibodies; Abs to Enzymes; Phospholipase

Applications Tested/Suitable for anti-PLCB3 antibody

Western Blot (WB)

Application Notes for anti-PLCB3 antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.

Other Notes

Small volumes of anti-PLCB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLCB3 purchase

MBS618384 is an antibody targeting PLCB3. Also known as Phospholipase C, beta 3, phosphorylated (Ser1105) (PLCb3). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PLCB3. It may not necessarily be applicable to this product.

NCBI GI #

829153

NCBI GeneID

5331

UniProt Primary Accession #

Q01970

UniProt Secondary Accession #

A5PKZ6

UniProt Related Accession #

Q01970; Q8N1A4

NCBI Official Full Name

phospholipase C beta 3

NCBI Official Synonym Full Names

phospholipase C, beta 3 (phosphatidylinositol-specific)

NCBI Official Symbol

PLCB3

NCBI Official Synonym Symbols

FLJ37084

UniProt Protein Name

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3

UniProt Synonym Protein Names

Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3

Protein Family

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase

UniProt Gene Name

PLCB3

UniProt Entry Name

PLCB3_HUMAN

NCBI Protein Information

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3; PLC beta 3; PLC-beta-3; OTTHUMP00000236934; OTTHUMP00000236935; OTTHUMP00000236936; phosphoinositide phospholipase C-beta-3

NCBI Summary for PLCB3

This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.

UniProt Comments for PLCB3

PLCB3: a phosphatidylinositol-specific phospholipase C. Binds to calmodulin. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate.

Protein type: Carbohydrate Metabolism - inositol phosphate; Phospholipase; EC 3.1.4.11

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; protein complex; membrane; cytosol

Molecular Function: calmodulin binding; signal transducer activity; calcium ion binding; phospholipase C activity; phosphoinositide phospholipase C activity

Biological Process: synaptic transmission; inositol phosphate metabolic process; regulation of systemic arterial blood pressure; lipid catabolic process

Research Articles on PLCB3

1. Activation of hPLCbeta3 by U73122 required covalent modification of cysteines as evidenced by the observation that enzyme activation was attenuated by thiol-containing nucleophiles, l-cysteine and glutathione.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PLCB3 antibody

Reference Product	PubMed Publications
PARD3 antibody	>1 publications with PLCB3 and PARD3

Pathways associated with anti-PLCB3 antibody

Products by Pathway	Pathway Diagram
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Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
CXCR4-mediated Signaling Events Pathway antibodies	CXCR4-mediated Signaling Events Pathway Diagram
Calcium Regulation In The Cardiac Cell Pathway antibodies	Calcium Regulation In The Cardiac Cell Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram

Diseases associated with anti-PLCB3 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>15 publications with PLCB3 and Neoplasms
Lung Diseases Antibodies	>4 publications with PLCB3 and Lung Diseases
Leukemia Antibodies	>3 publications with PLCB3 and Leukemia
Pain Antibodies	>3 publications with PLCB3 and Pain
Peritonitis Antibodies	>2 publications with PLCB3 and Peritonitis
Cardiomegaly Antibodies	>2 publications with PLCB3 and Cardiomegaly
Fibrosis Antibodies	>2 publications with PLCB3 and Fibrosis
Leukemia, Myeloid Antibodies	>2 publications with PLCB3 and Leukemia, Myeloid
Body Weight Changes Antibodies	>2 publications with PLCB3 and Body Weight Changes
Hyperplasia Antibodies	>1 publications with PLCB3 and Hyperplasia

Organs/Tissues associated with anti-PLCB3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>13 publications with PLCB3 and Brain
Lung Antibodies	>5 publications with PLCB3 and Lung
Uterus Antibodies	>3 publications with PLCB3 and Uterus
Bone Antibodies	>2 publications with PLCB3 and Bone
Connective Tissue Antibodies	>2 publications with PLCB3 and Connective Tissue
Skin Antibodies	>2 publications with PLCB3 and Skin
Kidney Antibodies	>2 publications with PLCB3 and Kidney
Testis Antibodies	>1 publications with PLCB3 and Testis
Embryonic Tissue Antibodies	>1 publications with PLCB3 and Embryonic Tissue
Intestine Antibodies	>1 publications with PLCB3 and Intestine