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C-Erbb2, P185 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS618482

c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor… More details

$970 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
c-erbB2, p185, Polyclonal Antibody
Full Product Name
c-erbB2, p185 (HER2, neu Receptor) (BSA & Azide Free)
Product Synonym Names
Anti -c-erbB2, p185 (HER2, neu Receptor) (BSA & Azide Free)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4. No stablilizing proteins and no preservatives added. Available with BSA and sodium azide. See C0015.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes wild and mutant (oncogenic) forms of human c-erbB-2 protein (185kD). Species Crossreactivity: monkey, rat and mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide derived from the C-terminus of human c-erbB-2/HER-2 protein. Sequence is identical in rat neu protein. Cellular Localization: Cell membrane. Epitope: C-terminal.
Positive Control
SKBR-3 cells, T47D cells or breast carcinomas.

Product Categories/Family for anti-c-erbB2, p185 antibody

Antibodies; Abs to Oncoproteins
Applications Tested/Suitable for anti-c-erbB2, p185 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-c-erbB2, p185 antibody
Suitable for ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry.
Dilution: Immunoprecipitation: 10ug/mg protein lysate. Use Protein A.
Western Blot: 5-10ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.

Western Blot (WB) of anti-c-erbB2, p185 antibody

Western Blot using MBS618482 human cells on left, mouse cells on right.
Rabbit Anti-C-Erbb2, P185 Polyclonal Antibody – Cat# MBS618482

Western Blot (WB) of anti-c-erbB2, p185 antibody

IP / Westem Blot of human cells using MBS618482
Rabbit Anti-C-Erbb2, P185 Polyclonal Antibody, Western Blot (WB) – Cat# MBS618482

Immunohistochemistry (IHC) of anti-c-erbB2, p185 antibody

Formalin-fixed, paraffin-embedded human breast cancer stained with MBS618482 using peroxidase labeled and AEC chromogen. Note membrane staining of tumor cells.
Rabbit Anti-C-Erbb2, P185 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS618482

Other Notes

Small volumes of anti-c-erbB2, p185 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for c-erbB2, p185 purchase

MBS618482 is an antibody targeting c-erbB2, p185. Also known as c-erbB2, p185 (HER2, neu Receptor) (BSA & Azide Free). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.
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