NBC1 Antibody – Rabbit Polyclonal

Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.

• 1. Romero, M.F., et al., (1998) Am. J. Physiol. 274, F425-F432. 3. Burnham, C.E., et al., (1998) gene accession # AF027362. 3. Burnham, C.E. et al., (1997) J. Biol. Chem. 272, 19111-19114. 4. Abuladze, N., et al., (1998) J. Biol. Chem. 273; 17689-17695. 5. Giffard, R.G., et al.,(2000) J. Neurochem. 6. Solemani, M. & Burnham, C.E., et al., (2000) Kidney Intl. 57, 371-384 (review). 7. Romero, M.F. & Boron, W.F., et al., (1999) Ann. Rev. Physiol. 61, 699-723 (review).

Small volumes of anti-NBC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS618636 is an antibody targeting NBC1. Also known as Anti - NBC1 (Na, HCO3 Cotransporter 1, pNBC). Reactive in Rat. Host: Rabbit
Source: Rat.