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Pi 4-Kinase, Beta (pi4kb) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS618657

Phosphatidylinositol (PI) 4-Kinases catalyze the first committed step in the biosynthesis of PI 4,5-biphosphate. PI 4-Kinases catalyze the phosphorylation of PI at the D-4 position of inositol ring. PI 4-phosphate is subsequently phosphorylated to form PI… More details

$795 / 0.2 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PI 4-Kinase, beta (pi4kb), Polyclonal Antibody
Full Product Name
PI 4-Kinase, beta
Product Gene Name
Product Synonym Names
Anti -PI 4-Kinase, beta
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a solution in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% Sodium Azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes wortmannin-sensitive human PI 4-Kinase beta. Species Crossreactivity: Mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50%) glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
90,455 Da
3D Structure
ModBase 3D Structure for Q6GN16
Immunogen
GST fusion protein corresponding to residues 410-537 of human PI 4-Kinase beta.
Positive Control
J9000
Jurkat cell lysate. Use 20ug per lane for minigels.
Applications Tested/Suitable for anti-pi4kb antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-pi4kb antibody
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 1-2ug/ml detected PI4-Kinase, beta in modified RIPA lysates of Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P4186-25 (1ug/ml). Proteins were visualized using a goat anti rabbit-IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 5ug immunoprecipitated PI 4-Kinase beta from 500ug of Jurkat RIPA lysate.
Immunocytochemistry: Reported to detect PI 4-Kinase beta in paraformaldehyde-fixed human platelets and the COS cell line.4

Other Notes

Small volumes of anti-pi4kb antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for pi4kb purchase

MBS618657 is an antibody targeting pi4kb. Also known as PI 4-Kinase, beta. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for pi4kb. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphatidylinositol 4-kinase beta
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase, catalytic, beta
NCBI Official Symbol
pi4kb
NCBI Official Synonym Symbols
pik4cb; MGC83640; PI4Kbeta; PI4K-beta
UniProt Protein Name
Phosphatidylinositol 4-kinase beta
UniProt Gene Name
pi4kb
UniProt Synonym Gene Names
pik4cb
UniProt Entry Name
PI4KB_XENLA
NCBI Protein Information
phosphatidylinositol 4-kinase beta; PtdIns 4-kinase beta; phosphatidylinositol 4-kinase, catalytic, beta polypeptide

UniProt Comments for pi4kb

Function: Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP)

Catalytic activity: ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.

Subcellular location: Endomembrane system

By similarity. Mitochondrion outer membrane; Peripheral membrane protein

By similarity. Rough endoplasmic reticulum membrane; Peripheral membrane protein

Sequence similarities: Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.Contains 1 PI3K/PI4K domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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