Rabbit Anti-RPS6KA1 Polyclonal Antibody – Cat# MBS619189

Western blot using Immunochemicals' affinity purified anti- p90 RSK1 pS732 antibody shows detection of a band ~90kD in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL

RPS6KA1 Antibody – Rabbit Polyclonal

Name: RPS6KA1 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS619189
Price: 1140 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS619189

Ribosomal S6 Kinase 1 (RSK1, S6K-alpha 1, 90kD Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, or MAPKAPK1A) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and… More details

$1140 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RSK1 (RPS6KA1), Polyclonal Antibody

Full Product Name

RSK1, phosphorylated (Ser732) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Prote

Product Gene Name

anti-RPS6KA1 antibody

Product Synonym Names

Anti -RSK1, phosphorylated (Ser732) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Prote

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human RSK1 when phosphorylated at Ser732. Species Sequence Homology: rat, mouse, horse, bovine, and dog..

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

~90kD

Chromosome Location

Chromosome: 1; NC_000001.10 (26856249..26901520). Location: 1p

OMIM

601684

3D Structure

ModBase 3D Structure for Q15418

Immunogen

Synthetic phosphopeptide corresponding to a region near the C-terminal end of human RSK1 protein.

Product Categories/Family for anti-RPS6KA1 antibody

Antibodies; Abs to Protein Kinases; Ribosomal

Applications Tested/Suitable for anti-RPS6KA1 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-RPS6KA1 antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:100,000
Western Blot: 1:500-1:2,000

Other Notes

Small volumes of anti-RPS6KA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPS6KA1 purchase

MBS619189 is an antibody targeting RPS6KA1. Also known as RSK1, phosphorylated (Ser732) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Prote. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPS6KA1. It may not necessarily be applicable to this product.

NCBI GI #

55743134

NCBI GeneID

6195

NCBI Accession #

NP_001006666.1

UniProt Primary Accession #

Q15418

UniProt Secondary Accession #

Q5SVM5; Q5SVM8; Q5SVM9; Q96C05; Q9BQK2; A6NGG4; A8K9K7; B2RDY8; B7Z5J0

UniProt Related Accession #

Q15418

GenBank Nucleotide #

NM_001006665.1

NCBI Official Full Name

ribosomal protein S6 kinase alpha-1 isoform b

NCBI Official Synonym Full Names

ribosomal protein S6 kinase, 90kDa, polypeptide 1

NCBI Official Symbol

RPS6KA1

NCBI Official Synonym Symbols

RSK; HU-1; RSK1; MAPKAPK1A

UniProt Protein Name

Ribosomal protein S6 kinase alpha-1

UniProt Synonym Protein Names

90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1

Protein Family

Ribosomal protein S6 kinase

UniProt Gene Name

RPS6KA1

UniProt Synonym Gene Names

MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1

UniProt Entry Name

KS6A1_HUMAN

NCBI Protein Information

ribosomal protein S6 kinase alpha-1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)

NCBI Summary for RPS6KA1

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

UniProt Comments for RPS6KA1

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Kinase, protein; EC 2.7.11.1; Translation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 1p

Cellular Component: nucleoplasm; cytoplasm; spindle; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; magnesium ion binding; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: regulation of transcription in response to stress; axon guidance; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; regulation of translation in response to stress; stress-activated MAPK cascade; negative regulation of caspase activity; toll-like receptor 3 signaling pathway; signal transduction; cell cycle; protein amino acid phosphorylation; positive regulation of cell growth; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; synaptic transmission; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

Research Articles on RPS6KA1

1. results indicate that RSK directly phosphorylates Gab2 on 3 serine residues; findings show RSK-mediated Gab2 phosphorylation inhibits Shp2 recruitment, suggesting RSK mediates a negative-feedback loop that attenuates Gab2-dependent functions including cell motility

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPS6KA1 antibody

Reference Product	PubMed Publications
MAPK1 antibody	>29 publications with RPS6KA1 and MAPK1
MAPK3 antibody	>27 publications with RPS6KA1 and MAPK3
RPS6 antibody	>6 publications with RPS6KA1 and RPS6
TSC2 antibody	>6 publications with RPS6KA1 and TSC2
EIF4B antibody	>5 publications with RPS6KA1 and EIF4B
TSC1 antibody	>4 publications with RPS6KA1 and TSC1
CREB1 antibody	>3 publications with RPS6KA1 and CREB1

Pathways associated with anti-RPS6KA1 antibody

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies	Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram
CREB Phosphorylation Pathway antibodies	CREB Phosphorylation Pathway Diagram
CREB Phosphorylation Through The Activation Of Ras Pathway antibodies	CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
Cytoplasmic Ribosomal Proteins Pathway antibodies	Cytoplasmic Ribosomal Proteins Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram

Diseases associated with anti-RPS6KA1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>33 publications with RPS6KA1 and Neoplasms
Inflammation Antibodies	>13 publications with RPS6KA1 and Inflammation
Hypertrophy Antibodies	>11 publications with RPS6KA1 and Hypertrophy
Disease Models, Animal Antibodies	>10 publications with RPS6KA1 and Disease Models, Animal
Liver Diseases Antibodies	>10 publications with RPS6KA1 and Liver Diseases
Cardiovascular Diseases Antibodies	>7 publications with RPS6KA1 and Cardiovascular Diseases
Breast Neoplasms Antibodies	>7 publications with RPS6KA1 and Breast Neoplasms
Hyperplasia Antibodies	>6 publications with RPS6KA1 and Hyperplasia
Adenocarcinoma Antibodies	>5 publications with RPS6KA1 and Adenocarcinoma
Cell Transformation, Neoplastic Antibodies	>5 publications with RPS6KA1 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-RPS6KA1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>45 publications with RPS6KA1 and Muscle
Blood Antibodies	>26 publications with RPS6KA1 and Blood
Brain Antibodies	>24 publications with RPS6KA1 and Brain
Liver Antibodies	>23 publications with RPS6KA1 and Liver
Heart Antibodies	>19 publications with RPS6KA1 and Heart
Lung Antibodies	>8 publications with RPS6KA1 and Lung
Connective Tissue Antibodies	>7 publications with RPS6KA1 and Connective Tissue
Vascular Antibodies	>7 publications with RPS6KA1 and Vascular
Embryonic Tissue Antibodies	>5 publications with RPS6KA1 and Embryonic Tissue
Bone Antibodies	>5 publications with RPS6KA1 and Bone