Sheep Anti-ENPP7 Polyclonal Antibody – Cat# MBS619220

Detection of ENPP-7 with MBS619220. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-mENPP-7.

Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP7) Antibody – Sheep Polyclonal

Name: ENPP7 Antibody; Polyclonal; Sheep; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS619220
Price: 1090 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS619220

ENPP7 is a member of the ecto-nucleotide pyrophosphatase/ phosphodiesterase (NPP) family with specific activity against lipids with positively charged phosphocholine headgroup including sphingomyelin, lysophosphatidylcholine and platelet activating factor… More details

$1090 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP7), Polyclonal Antibody

Full Product Name

Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP-7, Gm254, Alk-SMase, Enpp7)

Product Gene Name

anti-ENPP7 antibody

Product Synonym Names

Anti -Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP-7, Gm254, Alk-SMase, Enpp7)

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Species Reactivity

Mouse

Form/Format

Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes mouse ENPP-7. Species sequence homology: human (50%).

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

51,494 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (77704882..77716021). Location: 17q25.3

3D Structure

ModBase 3D Structure for Q6UWV6

Immunogen

NS0-derived, recombinant mouse ENPP-7.

Product Categories/Family for anti-ENPP7 antibody

Antibodies; Abs to Enzymes; Phosphatase

Applications Tested/Suitable for anti-ENPP7 antibody

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Application Notes for anti-ENPP7 antibody

Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml

Other Notes

Small volumes of anti-ENPP7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENPP7 purchase

MBS619220 is an antibody targeting ENPP7. Also known as Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP-7, Gm254, Alk-SMase, Enpp7). Reactive in Mouse. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for ENPP7. It may not necessarily be applicable to this product.

NCBI GI #

45545421

NCBI GeneID

339221

NCBI Accession #

NP_848638.2

UniProt Primary Accession #

Q6UWV6

UniProt Secondary Accession #

Q6ZTS5; Q8IUS8

UniProt Related Accession #

Q6UWV6

GenBank Nucleotide #

NM_178543.3

NCBI Official Full Name

ectonucleotide pyrophosphatase/phosphodiesterase family member 7

NCBI Official Synonym Full Names

ectonucleotide pyrophosphatase/phosphodiesterase 7

NCBI Official Symbol

ENPP7

NCBI Official Synonym Symbols

MGC50179; ALK-SMase

UniProt Protein Name

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7

UniProt Synonym Protein Names

Alkaline sphingomyelin phosphodiesterase; Intestinal alkaline sphingomyelinase

Protein Family

Ectonucleotide pyrophosphatase/phosphodiesterase family

UniProt Gene Name

ENPP7

UniProt Entry Name

ENPP7_HUMAN

NCBI Protein Information

ectonucleotide pyrophosphatase/phosphodiesterase family member 7; NPP-7; E-NPP 7; alkaline sphingomyelinase; intestinal alkaline sphingomyelinase; alkaline sphingomyelin phosphodiesterase

UniProt Comments for ENPP7

ENPP7: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Phosphodiesterase; EC 3.1.4.12; Membrane protein, integral; DNA replication; Cell cycle regulation; Lipid Metabolism - sphingolipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: Golgi apparatus; microvillus; membrane; integral to membrane; plasma membrane

Molecular Function: sphingomyelin phosphodiesterase activity

Biological Process: negative regulation of cell proliferation; sphingomyelin metabolic process; sphingolipid metabolic process; negative regulation of DNA replication; glycosphingolipid metabolic process; sphingomyelin catabolic process

Research Articles on ENPP7

1. predict the three-dimensional structure of NPP7 by homology modeling using a recently crystallized NPP from bacteria. Using the model, we studied the substrate specificity of the enzyme by docking.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ENPP7 antibody

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