Goat Anti-ATG4D Polyclonal Antibody – Cat# MBS619226

ATG4D Antibody – Goat Polyclonal

Name: ATG4D Antibody; Polyclonal; Goat; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS619226
Price: 835 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS619226

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and… More details

$835 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ATG4D, Polyclonal Antibody

Full Product Name

ATG4D (ATG4 Autophagy Related 4 Homolog D, PG4D, APG4-D, AUTL4, AUT-like 4 Cysteine Endopeptidase, Autophagin-4, Autophagy-related Cysteine Endopeptidase 4, Autophagy-related Protein 4 Homolog D, Cysteine Protease ATG4D)

Product Gene Name

anti-ATG4D antibody

Product Synonym Names

Anti -ATG4D (ATG4 Autophagy Related 4 Homolog D, PG4D, APG4-D, AUTL4, AUT-like 4 Cysteine Endopeptidase, Autophagin-4, Autophagy-related Cysteine Endopeptidase 4, Autophagy-related Protein 4 Homolog D, Cysteine Protease ATG4D)

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Human, Mouse, Rat

Form/Format

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human ATG4D at ~26.2kD. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kD. Species sequence homology: mouse, rat, canine, bovine, porcine.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 monthsat -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

16,008 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (10654647..10664094). Location: 19p13.2

3D Structure

ModBase 3D Structure for Q969K0

Immunogen

Synthetic peptide, CSQLAQPTLRLPRT from the internal region (near C-terminus) of human ATG4D.

Positive Control

Human Skeletal Muscle lysates

Product Categories/Family for anti-ATG4D antibody

Antibodies; Abs to Autophagy

Applications Tested/Suitable for anti-ATG4D antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-ATG4D antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA:1:32000
Western Blot:1ug/ml

Other Notes

Small volumes of anti-ATG4D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG4D purchase

MBS619226 is an antibody targeting ATG4D. Also known as ATG4D (ATG4 Autophagy Related 4 Homolog D, PG4D, APG4-D, AUTL4, AUT-like 4 Cysteine Endopeptidase, Autophagin-4, Autophagy-related Cysteine Endopeptidase 4, Autophagy-related Protein 4 Homolog D, Cysteine Protease ATG4D). Reactive in Human, Mouse, Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG4D. It may not necessarily be applicable to this product.

NCBI GI #

16877152

NCBI GeneID

84971

UniProt Primary Accession #

Q969K0

UniProt Related Accession #

Q53FA6; Q86TL0; Q969K0; Q96MZ6

NCBI Official Full Name

ATG4D protein

NCBI Official Synonym Full Names

ATG4 autophagy related 4 homolog D (S. cerevisiae)

NCBI Official Symbol

ATG4D

NCBI Official Synonym Symbols

APG4D; AUTL4; APG4-D

UniProt Protein Name

ATG4 autophagy related 4 homolog D (S. cerevisiae), isoform CRA_a

Protein Family

Cysteine protease

UniProt Gene Name

ATG4D

UniProt Entry Name

Q969K0_HUMAN

NCBI Protein Information

cysteine protease ATG4D; autophagin-4; APG4 autophagy 4 homolog D; AUT-like 4 cysteine endopeptidase; AUT-like 4, cysteine endopeptidase; autophagy-related protein 4 homolog D; cysteine protease involved in autophagy; autophagy-related cysteine endopeptidase 4

UniProt Comments for ATG4D

ATG4D: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Belongs to the peptidase C54 family.

Protein type: Protease; Autophagy; EC 3.4.22.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: mitochondrial matrix; cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity

Biological Process: protein delipidation; apoptosis; mitochondrion degradation; protein processing; C-terminal protein lipidation; protein targeting to membrane; proteolysis; cellular response to nitrogen starvation; autophagic vacuole formation

Research Articles on ATG4D

1. Caspase cleavage of Atg4D stimulates GABARAP-L1 processing and triggers mitochondrial targeting and apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ATG4D antibody

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