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PDX1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS619404

PDX-1 (Pancreas-duodenum homeobox 1; also IPF-1, IDX-1, STF-1, GSF and IUF-1) is a 42kD member of the IPF-1 subfamily, ANTP homeobox family of proteins. It is expressed in developing pancreas, plus pancreatic beta-cells, and duodenal crypt epithelium. In… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Pdx1, Polyclonal Antibody
Full Product Name
Pdx1 (Pdx Family Homeodomain Transcription Factor, Pancreas/Duodenum Homeobox 1, Glucose Sensitive Factor, GSF, Insulin Promoter Factor 1, IPF1, Islet/Duodenum Homeobox 1, IDX1, Insulin Upstream Factor 1, IUF1, MODY4, Somatostatin Transactivating Factor 1
Product Gene Name
Product Synonym Names
Anti -Pdx1 (Pdx Family Homeodomain Transcription Factor, Pancreas/Duodenum Homeobox 1, Glucose Sensitive Factor, GSF, Insulin Promoter Factor 1, IPF1, Islet/Duodenum Homeobox 1, IDX1, Insulin Upstream Factor 1, IUF1, MODY4, Somatostatin Transactivating Factor 1
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Mouse, Rat
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Pdx1. Species Crossreactivity: rat.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
45,362 Da
3D Structure
ModBase 3D Structure for P16451
Immunogen
Purified, E. coli-derived recombinant mouse Pancrease/Duodenum Homeobox-1 (aa1-284; Accession # P52946).
Applications Tested/Suitable for anti-PDX1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-PDX1 antibody
Suitable for use in ELISA, Immunocytochemistry, and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunocytochemistry: 10ug/ml

SDS-Page of anti-PDX1 antibody

Detection of PDX-1 with MBS619404. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-PDX-1.
Goat Anti-PDX1 Polyclonal Antibody – Cat# MBS619404

Other Notes

Small volumes of anti-PDX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDX1 purchase

MBS619404 is an antibody targeting PDX1. Also known as Pdx1 (Pdx Family Homeodomain Transcription Factor, Pancreas/Duodenum Homeobox 1, Glucose Sensitive Factor, GSF, Insulin Promoter Factor 1, IPF1, Islet/Duodenum Homeobox 1, IDX1, Insulin Upstream Factor 1, IUF1, MODY4, Somatostatin Transactivating Factor 1. Reactive in Mouse, Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for PDX1. It may not necessarily be applicable to this product.

NCBI GI #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
PDX1
UniProt Protein Name
Pyruvate dehydrogenase complex protein X component, mitochondrial
UniProt Synonym Protein Names
Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; E3-binding protein; Pyruvate dehydrogenase complex component E3BP
UniProt Gene Name
PDX1
UniProt Entry Name
ODPX_YEAST

UniProt Comments for PDX1

Function: Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.

Subunit structure: Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.

Subcellular location: Mitochondrion matrix.

Miscellaneous: Present with 414 molecules/cell in log phase SD medium. Ref.7

Sequence similarities: Belongs to the 2-oxoacid dehydrogenase family.Contains 1 lipoyl-binding domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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