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PAK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS619619

The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor-induced neurite… More details

$830 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PAK1,2, Polyclonal Antibody
Full Product Name
PAK1,2, phosphorylated (Ser144/141)
Product Synonym Names
Anti -PAK1,2, phosphorylated (Ser144/141)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Guinea Pig, Human, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Ser144 phosphorylated PAK1 and Ser141 phosphorylated PAK2. This antibody also recognizes Ser139 phosphorylated PAK3 but does not cross-react with phosphorylated PAK4-6. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser144 of human PAK1 (KLH).

Product Categories/Family for anti-PAK1,2 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-PAK1,2 antibody
Western Blot (WB)
Application Notes for anti-PAK1,2 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.

Western Blot (WB) of anti-PAK1,2 antibody

Western blot analysis of extracts from COS cells cotransfected with activated cdc42 and GST-PAK2 (lane 1), transfected with GST-PAK2 alone (lane 2) or activated cdc42 alone (lane 3), using MBS619619 PAK1 Phosphorylated (Ser144)/PAK2 (Ser141) Antibody (upper) or PAK1/2/3 Antibody MBS619619 (lower). This antibody detects GST-PAK2 signal only when it is cotransfected with activated cdc42, indicating that this antibody is phospho-specific.
Rabbit Anti-PAK1 Polyclonal Antibody – Cat# MBS619619

Western Blot (WB) of anti-PAK1,2 antibody

Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP for indicated times, using P2488-1 PAK1 Phosphorylated (Ser144)/PAK2 (Ser141) Antibody (upper) and PAK1/2/3 Antibody MBS619619 (lower).
Rabbit Anti-PAK1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS619619

Other Notes

Small volumes of anti-PAK1,2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAK1,2 purchase

MBS619619 is an antibody targeting PAK1,2. Also known as PAK1,2, phosphorylated (Ser144/141). Reactive in Guinea Pig, Human, Mouse, Rat. Host: Rabbit.
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