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CBS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6199922

MBS6199922 is an antibody targeting CBS. Also known as CBS (Cystathionine beta-synthase, Serine Sulfhydrase, Beta-thionase) (MaxLight 490). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CBS, Monoclonal Antibody
Full Product Name
CBS (Cystathionine beta-synthase, Serine Sulfhydrase, Beta-thionase) (MaxLight 490)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3E1
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human CBS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
61,863 Da
OMIM
phenotype 613381
3D Structure
ModBase 3D Structure for P35520
Immunogen
Partial recombinant corresponding to aa1-100 from human CBS (NP_000062) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEPLWIRPDAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFG
Conjugate
MaxLight490

Product Categories/Family for anti-CBS antibody

Applications Tested/Suitable for anti-CBS antibody
FLISA, Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-CBS antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Product References and Citations for anti-CBS antibody

1. Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe. Dufton N, Natividad J, Verdu EF, Wallace JL.Sci Rep. 2012;2:499. Epub 2012 Jul 9. 2. Characterization of Hydrogen Sulfide and Its Synthases, Cystathionine ?]-Synthase and Cystathionine ?^-Lyase, in Human Prostatic Tissue and Cells. Guo H, Gai JW, Wang Y, Jin HF, Du JB, Jin J.Urology. 2012 Feb;79(2):483.e1-5. 3. Homocystinuria in Taiwan: An inordinately high prevalence in an Austronesian aboriginal tribe, Tao. Lu YH, Huang YH, Cheng LM, Yu HC, Hsu JH, Wu JT, Lo MY, Lin A, Lin CY, Wu JY, Niu DM.Molecular Genetics and Metabolism (2012), doi: 10.1016/j. ymgme.2012.01.021 4. Hydrogen Sulfide in the RVLM and PVN has No Effect on Cardiovascular Regulation. Streeter E, Al-Magableh M, Hart JL, Badoer E.Front Physiol. 2011;2:55. Epub 2011 Sep 1. 5. Interdependency of Cystathione ?^-Lyase and Cystathione ?]-Synthase in Hydrogen Sulfide-Induced Blood Pressure Regulation in Rats. Roy A, Khan AH, Islam MT, Prieto MC, Majid DS.Am J Hypertens. 2011 Aug 25. doi: 10.1038/ajh.2011.149. 6. Placental markers of folate-related metabolism in preeclampsia. Mislanova C, Martsenyuk O, Huppertz B, Obolenskaya MY.Reproduction. 2011 Jun 20. 7. Multiple hemodynamic effects of endogenous hydrogen sulfide on central nervous system in rats. Ren YS, Wu SY, Wang XJ, Yu F, Zhao J, Tang CS, Ouyang JP, Geng B.Chin Med J 2011;124(21):3468-3475. 8. Hydrogen Sulfide Modulates Contractile Function in Rat Jejunum. Kasparek MS, Linden DR, Farrugia G, Sarr MG.J Surg Res. 2011 Apr 22. 9. Carbon monoxide stimulates global protein methylation via its inhibitory action on cystathionine beta-synthase. Yamamoto T, Takano N, Ishiwata K, Suematsu M.J. Clin. Biochem. Nutr., 48, 96-100, 2010. 10. The hydrogen sulfide signaling system: changes during aging and the benefits of caloric restriction. Predmore BL, Alendy MJ, Ahmed KI, Leeuwenburgh C, Julian D.Age (Dordr). 2010 Dec;32(4):467-81. Epub 2010 May 26. 11. A Crucial Role for Hydrogen Sulfide in Oxygen Sensing via Modulating Large Conductance Calcium-Activated Potassium Channels. Li Q, Sun B, Wang X, Jin Z, Zhou Y, Dong L, Jiang LH, Rong W.Antioxid Redox Signal. 2010 May 15;12(10):1179-89. 12. Rescue of cystathionine beta-synthase (CBS) mutants with chemical chaperones: purification and characterization of eight CBS mutant enzymes. Majtan T, Liu L, Carpenter JF, Kraus JP.J Biol Chem. 2010 Mar 22. 13. Actions of hydrogen sulphide on ion transport across rat distal colon. Hennig B, Diener M.Br J Pharmacol. 2009 Nov;158(5):1263-75. Epub 2009 Sep 25. 14. The endogenous hydrogen sulfide producing enzyme cystathionine-?] synthase contributes to visceral hypersensitivity in a rat model of irritable bowel syndrome. Xu GY, Winston JH, Shenoy M, Zhou S, Chen JD, Pasricha PJ.Mol Pain. 2009 Aug 6;5:44. 15. Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide. Lee M, Schwab C, Yu S, McGeer E, McGeer PL.Neurobiol Aging. 2009 Oct;30(10):1523-34. Epub 2009 Jul 23. 16. Hydrogen sulphide synthesis in the rat and mouse gastrointestinal tract. Martin GR, McKnight GW, Dicay MS, Coffin CS, Ferraz JG, Wallace JL.Dig Liver Dis. 2009 Jun 29. 17. Endogenous and Exogenous Hydrogen Sulfide Promotes Resolution of Colitis in Rats. Wallace JL, Vong L, McKnight W, Dicay M, Martin GR.Gastroenterology. 2009 Aug;137(2):569-78, 578.e1. Epub 2009 Apr 16. 18. Active CBS can be expressed in heme-free systems in the presence of metal-substituted porphyrins or a chemical chaperone. Majtan T, Singh LR, Wang L, Kruger WD, Kraus JP.J Biol Chem. 2008 Dec 12;283(50):34588-95. Epub 2008 Oct 10. 19. Hydrogen sulfide enhances ulcer healing in rats. Wallace JL, Dicay M, McKnight W, Martin GR.FASEB J. 2007 Dec;21(14):4070-6. Epub 2007 Jul 18. 20. Hydrogen sulfide is a novel prosecretory neuromodulator in the Guinea-pig and human colon. Schicho R, Krueger D, Zeller F, Von Weyhern CW, Frieling T, Kimura H, Ishii I, De Giorgio R, Campi B, Schemann M.Gastroenterology. 2006 Nov;131(5):1542-52. Epub 2006 Aug 18.

Other Notes

Small volumes of anti-CBS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CBS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cystathionine beta-synthase
NCBI Official Synonym Full Names
cystathionine-beta-synthase
NCBI Official Symbol
CBS
NCBI Official Synonym Symbols
HIP4
UniProt Protein Name
Cystathionine beta-synthase
UniProt Synonym Protein Names
Beta-thionase; Serine sulfhydrase
UniProt Gene Name
CBS
UniProt Entry Name
CBS_HUMAN
NCBI Protein Information
cystathionine beta-synthase; beta-thionase; methylcysteine synthase; serine sulfhydrase

UniProt Comments for CBS

CBS: Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury. Defects in CBS are the cause of cystathionine beta- synthase deficiency (CBSD). CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine. Belongs to the cysteine synthase/cystathionine beta- synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Other Amino Acids Metabolism - selenoamino acid; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - glycine, serine and threonine; Lyase; EC 4.2.1.22

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: intracellular membrane-bound organelle; nucleolus; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; enzyme binding; cystathionine beta-synthase activity; ubiquitin protein ligase binding; metal ion binding; heme binding; pyridoxal phosphate binding

Biological Process: cysteine biosynthetic process via cystathionine; homocysteine catabolic process; sulfur amino acid metabolic process; L-serine metabolic process; transsulfuration; cysteine biosynthetic process from serine; homocysteine metabolic process; L-serine catabolic process; L-cysteine catabolic process

Disease: Homocystinuria Due To Cystathionine Beta-synthase Deficiency
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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