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CELA3A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6200078

MBS6200078 is an antibody targeting CELA3A. Also known as CELA3A (ELA3, ELA3A, Chymotrypsin-like Elastase Family Member 3A, Elastase IIIA, Elastase-3A, Protease E) (MaxLight 490). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CELA3A, Monoclonal Antibody
Full Product Name
CELA3A (ELA3, ELA3A, Chymotrypsin-like Elastase Family Member 3A, Elastase IIIA, Elastase-3A, Protease E) (MaxLight 490)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3G4
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human CELA3A.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
3D Structure
ModBase 3D Structure for P09093
Immunogen
Full length recombinant protein corresponding to aa16-270 from CELA3A with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
SGYGPPSSHSSSRVVHGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISRDLTYQVVLGEYNLAVKEGPEQVIPINSEELFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNKTPCYITGWGRLYTNGPLPDKLQQARLPVVDYKHCSRWNWWGSTVKKTMVCAGGYIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSGFGCNFIWKPTVFTRVSAFIDWIEETIASH
Conjugate
MaxLight490

Product Categories/Family for anti-CELA3A antibody

Applications Tested/Suitable for anti-CELA3A antibody
FLISA, Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-CELA3A antibody
Applications are based on unconjugated antibody.

Testing Data of anti-CELA3A antibody

Detection limit for recombinant GST tagged CELA3A is ~1ng/ml using MBS6005575 as a capture antibody.
Mouse Anti-CELA3A Monoclonal Antibody – Cat# MBS6200078

Western Blot (WB) of anti-CELA3A antibody

Western Blot detection against Immunogen using MBS6005575 (54.16kD).
Mouse Anti-CELA3A Monoclonal Antibody, Western Blot (WB) – Cat# MBS6200078

Other Notes

Small volumes of anti-CELA3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CELA3A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens elastase 3A, pancreatic, mRNA
NCBI Official Synonym Full Names
chymotrypsin like elastase 3A
NCBI Official Symbol
CELA3A
NCBI Official Synonym Symbols
ELA3; ELA3A
UniProt Protein Name
Chymotrypsin-like elastase family member 3A
UniProt Synonym Protein Names
Elastase IIIA; Elastase-3A; Protease E
UniProt Gene Name
CELA3A
UniProt Synonym Gene Names
ELA3; ELA3A
UniProt Entry Name
CEL3A_HUMAN
NCBI Protein Information
chymotrypsin-like elastase family member 3A

NCBI Summary for CELA3A

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008]
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UniProt Comments for CELA3A

CELA3A: Efficient protease with alanine specificity but only little elastolytic activity. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: EC 3.4.21.70; Protease

Chromosomal Location of Human Ortholog: 1p36.12

Molecular Function: serine-type endopeptidase activity

Biological Process: cholesterol metabolic process; digestion; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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