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Vaccinia Related Kinase 3 (VRK3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620008

The vaccinia-related kinase (VRK) proteins are a new group of Ser/Thr kinases in the human kinome. This mammalian kinase family comprises three members, VRK1, VRK2, and VRK3 (1-3). The VRK1 has autophosphorylation activity and phosphorylates several… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Vaccinia Related Kinase 3 (VRK3), Polyclonal Antibody
Full Product Name
Vaccinia Related Kinase 3 (VRK3)
Product Gene Name
Product Synonym Names
Anti -Vaccinia Related Kinase 3 (VRK3)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Purity/Purification
Purified by protein A and peptide affinity chromatography.
Specificity
Detects endogenous levels of total VRK3 protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
52,881 Da
Chromosome Location
chromosome: 19; Location: 19q13
3D Structure
ModBase 3D Structure for Q8IV63
Immunogen
Synthetic peptide (KLH-coupled) corresponding to human VRK3.
Applications Tested/Suitable for anti-VRK3 antibody
Western Blot (WB)
Application Notes for anti-VRK3 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000

Western Blot (WB) of anti-VRK3 antibody

Western blot analysis of extracts from Caki, C2C12 and COS cells using MBS620008.
Rabbit Anti-VRK3 Polyclonal Antibody – Cat# MBS620008

Other Notes

Small volumes of anti-VRK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VRK3 purchase

MBS620008 is an antibody targeting VRK3. Also known as Vaccinia Related Kinase 3 (VRK3). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VRK3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
vaccinia virus B1R kinase related kinase 3
NCBI Official Synonym Full Names
vaccinia related kinase 3
NCBI Official Symbol
VRK3
NCBI Official Synonym Symbols
VRK3
UniProt Protein Name
Inactive serine/threonine-protein kinase VRK3
UniProt Synonym Protein Names
Serine/threonine-protein pseudokinase VRK3; Vaccinia-related kinase 3
UniProt Gene Name
VRK3
UniProt Entry Name
VRK3_HUMAN
NCBI Protein Information
serine/threonine-protein kinase VRK3; vaccinia-related kinase 3

NCBI Summary for VRK3

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq]
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UniProt Comments for VRK3

VRK3: Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus. Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily. 2 human isoforms produced by alternative splicing.

Protein type: Protein kinase, CK1; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CK1 group; VRK family

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; ATP binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; regulation of cell shape; peptidyl-serine phosphorylation; Wnt receptor signaling pathway; endocytosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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