Sheep Anti-CHST1 Polyclonal Antibody – Cat# MBS620110

Detection of CHST1. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml sheep anti-hCHST1.

Carbohydrate Sulfotransferase 1 (CHST1) Antibody – Sheep Polyclonal

Name: CHST1 Antibody; Polyclonal; Sheep; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS620110
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620110

The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate… More details

$1105 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Carbohydrate Sulfotransferase 1 (CHST1), Polyclonal Antibody

Full Product Name

Carbohydrate Sulfotransferase 1 (CHST1, Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, GST-1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, K

Product Gene Name

anti-CHST1 antibody

Product Synonym Names

Anti -Carbohydrate Sulfotransferase 1 (CHST1, Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, GST-1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, K

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human CHST1.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

46,715 Da

Chromosome Location

Chromosome: 11; NC_000011.9 (45670427..45687172, complement). Location: 11p11.2

OMIM

603797

3D Structure

ModBase 3D Structure for O43916

Immunogen

CHO cell-derived, recombinant human CHST1 aa24- 411.

Product Categories/Family for anti-CHST1 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

Applications Tested/Suitable for anti-CHST1 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-CHST1 antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml

Other Notes

Small volumes of anti-CHST1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHST1 purchase

MBS620110 is an antibody targeting CHST1. Also known as Carbohydrate Sulfotransferase 1 (CHST1, Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, GST-1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, K. Reactive in Human. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for CHST1. It may not necessarily be applicable to this product.

NCBI GI #

4502841

NCBI GeneID

8534

NCBI Accession #

NP_003645.1

UniProt Primary Accession #

O43916

UniProt Secondary Accession #

D3DQP2

UniProt Related Accession #

O43916

GenBank Nucleotide #

NM_003654.4

NCBI Official Full Name

carbohydrate sulfotransferase 1

NCBI Official Synonym Full Names

carbohydrate (keratan sulfate Gal-6) sulfotransferase 1

NCBI Official Symbol

CHST1

NCBI Official Synonym Symbols

C6ST; KS6ST; KSGal6ST

UniProt Protein Name

Carbohydrate sulfotransferase 1

UniProt Synonym Protein Names

Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase

Protein Family

Carbohydrate sulfotransferase

UniProt Gene Name

CHST1

UniProt Entry Name

CHST1_HUMAN

NCBI Protein Information

carbohydrate sulfotransferase 1; KSST; GST-1; OTTHUMP00000233116; keratan sulfate Gal-6 sulfotransferase; carbohydrate (chondroitin 6/keratan) sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1

NCBI Summary for CHST1

This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]

UniProt Comments for CHST1

CHST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N- acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: EC 2.8.2.21; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: sulfotransferase activity; keratan sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; galactose metabolic process; pathogenesis; polysaccharide metabolic process; inflammatory response

Research Articles on CHST1

1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CHST1 antibody

Products by Pathway	Pathway Diagram
Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies	Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies	Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram

Diseases associated with anti-CHST1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>2 publications with CHST1 and Inflammation
Drug Toxicity Antibodies	>1 publications with CHST1 and Drug Toxicity

Organs/Tissues associated with anti-CHST1 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>1 publications with CHST1 and Lung
Eye Antibodies	>1 publications with CHST1 and Eye
Brain Antibodies	>1 publications with CHST1 and Brain