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Heterochromatin Protein 1, Alpha (LOC100689444) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620124

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding… More details

$835 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Heterochromatin Protein 1, alpha (LOC100689444), Polyclonal Antibody
Full Product Name
Heterochromatin Protein 1, alpha (HP1a, Antigen p25, Chromobox Homolog 5 (Drosophila), Cbx5, HP1Hs-alpha)
Product Synonym Names
Anti -Heterochromatin Protein 1, alpha (HP1a, Antigen p25, Chromobox Homolog 5 (Drosophila), Cbx5, HP1Hs-alpha)
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Specificity
Recognizes human CBX5 at ~22kD. Species sequence homology: mouse, canine, chinese hamster.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 monthsat -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide, C-NKRKSNFSNSADDIK, from the internal region of human CBX5.
Positive Control
Human fibroblast A431 and HeLa cell lysates.
Applications Tested/Suitable for anti-LOC100689444 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-LOC100689444 antibody
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Dilution: ELISA: 1:128,000
Immunohistochemistry (paraffin): 2-3ug/ml
Western Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher

Immunohistochemistry (IHC) of anti-LOC100689444 antibody

MBS620124 (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-LOC100689444 Polyclonal Antibody – Cat# MBS620124

Chemiluminescence of anti-LOC100689444 antibody

MBS620124 (0.03ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-LOC100689444 Polyclonal Antibody, Chemiluminescence – Cat# MBS620124

Other Notes

Small volumes of anti-LOC100689444 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LOC100689444 purchase

MBS620124 is an antibody targeting LOC100689444. Also known as Heterochromatin Protein 1, alpha (HP1a, Antigen p25, Chromobox Homolog 5 (Drosophila), Cbx5, HP1Hs-alpha). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for LOC100689444. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
heterochromatin protein 1 alpha
NCBI Official Symbol
LOC100689444
NCBI Protein Information
heterochromatin protein 1 alpha

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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