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IgG, F(ab')2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620336

MBS620336 is an antibody targeting IgG, F(ab')2. Also known as IgG, F(ab')2 (AP). Reactive in Guinea Pig. Host: Goat. More details

$540 / 0.5 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG, F(ab')2, Polyclonal Antibody
Full Product Name
IgG, F(ab')2 (AP)
Product Synonym Names
Anti -IgG, F(ab')2 (AP)
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Guinea Pig
Form/Format
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Labeled with Alkaline phosphatase (AP).
Purity/Purification
Affinity Purified
Purified by immunoaf nity chromatography.
Specificity
Recognizes guinea pig IgG, F(ab')2. Assay by IEP resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(c).
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.
Immunogen
Guinea Pig IgG F(ab')2 fragment
Applications Tested/Suitable for anti-IgG, F(ab')2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IgG, F(ab')2 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Capture ELISA: 1:2000-1:15,000. Assayed against 1ug of Guinea Pig IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT.
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000

Other Notes

Small volumes of anti-IgG, F(ab')2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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