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IgG, F(ab')2, X-Adsorbed Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620567

MBS620567 is an antibody targeting IgG, F(ab')2, X-Adsorbed. Also known as IgG, F(ab')2, X-Adsorbed (PE). Reactive in Mouse. Host: Goat. More details

$435 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG, F(ab')2, X-Adsorbed, Polyclonal Antibody
Full Product Name
IgG, F(ab')2, X-Adsorbed (PE)
Product Synonym Names
Anti -IgG, F(ab')2, X-Adsorbed (PE)
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA (IgG and Protease free), 0.05% sodium azide, 40% glycerol. Labeled with R-Phycoerythrin (R-PE).
Purity/Purification
Affinity Purified
Purified by immunoaf nity chromatography.
Specificity
Recognizes Mouse IgG F(ab')2. Assay by IEP resulted in a single precipitin arc against anti-phycoerythrin, anti-goat serum, mouse IgG, mouse IgG F(ab')2 and mouse serum. Does not crossreact with anti-pepsin, anti-goat IgG F(c), mouse IgG F(c) or bovine, equine or human serum proteins.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mouse IgG F(ab')2 fragment.
Label
R-Phycoerythrin (R-PE)
MW: 240kD.
Absorption Wavelength: 490nm, 545nm and 565nm.
Emission Wavelength: 580nm.
Phycobiliprotein Concentration: 0.5mg/ml; Determined by absorbance = 82.0 at 565nm for a 1% solution for R-PE molecules to which at least one molecule of active antibody is bound.
Applications Tested/Suitable for anti-IgG, F(ab')2, X-Adsorbed antibody
ELISA (EL/EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Suitable for use in ELISA, Immunofluorescence and Flow Cytometry.
Dilution: Immunofluorescence: 1:100-1:250
Flow Cytometry: 1:100-1:250 (~1ug will stain 1x10e6 cells)

Other Notes

Small volumes of anti-IgG, F(ab')2, X-Adsorbed antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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