• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LOC396473 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620589

Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC. MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth. Through its basic effector region, MARCKS associates with acidic… More details

$895 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MARCKS (LOC396473), Polyclonal Antibody
Full Product Name
MARCKS, phosphorylated (Ser152/156) (myristoylated alanine-rich protein kinase C substrate, 80K-L, 80K-L Protein, FLJ14368, FLJ90045, MACS, Myristoylated alanine-rich C-kinase substrate, PKCSL, PRKCSL, Protein kinase C substrate, 80kD Protein, Light Chain
Product Gene Name
anti-LOC396473 antibody
Product Synonym Names
Anti -MARCKS, phosphorylated (Ser152/156) (myristoylated alanine-rich protein kinase C substrate, 80K-L, 80K-L Protein, FLJ14368, FLJ90045, MACS, Myristoylated alanine-rich C-kinase substrate, PKCSL, PRKCSL, Protein kinase C substrate, 80kD Protein, Light Chain
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM sodium chloride, 100ug/mL BSA, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat MARCKS when phosphorylated at Ser152/156.. Species Crossreactivity: mouse, human.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
27,728 Da
3D Structure
ModBase 3D Structure for P16527
Immunogen
Synthetic phosphopeptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS.
Positive Control
A431 Cell Lysate

Product Categories/Family for anti-LOC396473 antibody

Antibodies; Abs to Protein Kinase Substrates
Applications Tested/Suitable for anti-LOC396473 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-LOC396473 antibody
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:500
Immunohistochemistry (paraffin): 1:100

Immunohistochemistry (IHC) of anti-LOC396473 antibody

Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta MARCKS representative staining pattern/morphology in rat placenta. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.
Rabbit Anti-LOC396473 Polyclonal Antibody – Cat# MBS620589

Western Blot (WB) of anti-LOC396473 antibody

Western Blot Analysis: EGF treated A431 lysates were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-MARCKS PHOSPHO SER152/156 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MARCKS (~80 kD).
Rabbit Anti-LOC396473 Polyclonal Antibody, Western Blot (WB) – Cat# MBS620589

Western Blot (WB) of anti-LOC396473 antibody

Western Blot Analysis: Representative image from a previous lot. pervanadate treated HEK293 lysate (Lane 1), pervanadate treated C2C12 lysate (Lane 2), pervanadate treated Huvec lysate (Lane 3), EGF treated A431 lysate (Lane 4), pervanadate treated L6 lysate (Lane 5) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-MARCKS PHOSPHO SER152/156 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated MARCKS (~80kD).
Rabbit Anti-LOC396473 Polyclonal Antibody, Western Blot (WB) – Cat# MBS620589

Other Notes

Small volumes of anti-LOC396473 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LOC396473 purchase

MBS620589 is an antibody targeting LOC396473. Also known as MARCKS, phosphorylated (Ser152/156) (myristoylated alanine-rich protein kinase C substrate, 80K-L, 80K-L Protein, FLJ14368, FLJ90045, MACS, Myristoylated alanine-rich C-kinase substrate, PKCSL, PRKCSL, Protein kinase C substrate, 80kD Protein, Light Chain. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LOC396473. It may not necessarily be applicable to this product.

NCBI GI #
45382723
NCBI GeneID
396473
NCBI Accession #
NP_990811.1
UniProt Primary Accession #
P16527
UniProt Related Accession #
P16527
GenBank Nucleotide #
NM_205480.1
NCBI Official Full Name
myristoylated alanine-rich C-kinase substrate
NCBI Official Symbol
LOC396473
UniProt Protein Name
Myristoylated alanine-rich C-kinase substrate
Protein Family
MARCKS-related protein
UniProt Gene Name
MARCKS
UniProt Entry Name
MARCS_CHICK
NCBI Protein Information
myristoylated alanine-rich C-kinase substrate; MARCKS

UniProt Comments for LOC396473

MARCKS: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein

Protein type: Motility/polarity/chemotaxis; Actin-binding

Cellular Component: cytoskeleton; membrane; cytoplasm

Molecular Function: calmodulin binding; actin binding
Show more

Research Articles on LOC396473

1. these results indicate the existence of a functional interaction between actin filaments and MARCKS in cells, and particularly of an action in maintaining a phosphorylation in a region of the N-terminal moiety of MARCKS.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-LOC396473 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >79 publications with LOC396473 and Neoplasms
 Nervous System Diseases Antibodies  >35 publications with LOC396473 and Nervous System Diseases
 Inflammation Antibodies  >19 publications with LOC396473 and Inflammation
 Carcinoma Antibodies  >16 publications with LOC396473 and Carcinoma
 Disease Models, Animal Antibodies  >15 publications with LOC396473 and Disease Models, Animal
 Immune System Diseases Antibodies  >14 publications with LOC396473 and Immune System Diseases
 Necrosis Antibodies  >12 publications with LOC396473 and Necrosis
 Cell Transformation, Neoplastic Antibodies  >12 publications with LOC396473 and Cell Transformation, Neoplastic
 Depressive Disorder Antibodies  >12 publications with LOC396473 and Depressive Disorder
 Cardiovascular Diseases Antibodies  >11 publications with LOC396473 and Cardiovascular Diseases

Organs/Tissues associated with anti-LOC396473 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >214 publications with LOC396473 and Brain
 Muscle Antibodies  >44 publications with LOC396473 and Muscle
 Embryonic Tissue Antibodies  >19 publications with LOC396473 and Embryonic Tissue
 Liver Antibodies  >14 publications with LOC396473 and Liver
 Head Antibodies  >12 publications with LOC396473 and Head
 Ovary Antibodies  >8 publications with LOC396473 and Ovary
 Gonad Antibodies  >8 publications with LOC396473 and Gonad
 Limb Antibodies  >5 publications with LOC396473 and Limb
 Spleen Antibodies  >3 publications with LOC396473 and Spleen
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000