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IgG, F(ab')2 X-Adsorbed Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620610

MBS620610 is an antibody targeting IgG, F(ab')2 X-Adsorbed. Also known as IgG, F(ab')2 X-Adsorbed (Biotin). Reactive in Human. Host: Rabbit. More details

$810 / 1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG, F(ab')2 X-Adsorbed, Polyclonal Antibody
Full Product Name
IgG, F(ab')2 X-Adsorbed (Biotin)
Product Synonym Names
Anti -IgG, F(ab')2 X-Adsorbed (Biotin)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA
Purity/Purification
Affinity Purified
Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human IgG F(ab')2 fragment
Label
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio
10-20 BAC molecules per Rabbit IgG molecule
Applications Tested/Suitable for anti-IgG, F(ab')2 X-Adsorbed antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Suitable foruse in Immunoblot, ELISA, Immunohistochemistry, Immunomicroscopy.
Dilution: Standard Capture ELISA: 1:12,000 to 1:55,000, 1ug of Human IgG in a using Peroxidase Conjugated Streptavidin.
Optimal dilution determined by the researcher.

Other Notes

Small volumes of anti-IgG, F(ab')2 X-Adsorbed antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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