Mouse Anti-HERC3 Monoclonal Antibody – Cat# MBS6206592

HERC3 Antibody – Mouse Monoclonal

Name: HERC3 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS6206592
Price: 1140 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6206592

HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before… More details

$1140 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HERC3, Monoclonal Antibody

Full Product Name

HERC3 (hect Domain and RLD 3, KIAA0032) (MaxLight 490)

Product Gene Name

anti-HERC3 antibody

Product Synonym Names

hect Domain and RLD 3; KIAA0032

Clonality

Monoclonal

Isotype

IgG1,k

Clone Number

3G7

Host

Mouse

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

Purity/Purification

Purified

Specificity

Recognizes HERC3.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

OMIM

D25215 mRNA

3D Structure

ModBase 3D Structure for Q15034

Immunogen

HERC3 (NP_055421, 1aa-109aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.

Immunogen Sequence

MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWG

Conjugate

MaxLight490

Product Categories/Family for anti-HERC3 antibody

Antibodies; Enzymes

Applications Tested/Suitable for anti-HERC3 antibody

FLISA, Western Blot (WB)

Application Notes for anti-HERC3 antibody

Applications are based on unconjugated antibody.

Other Notes

Small volumes of anti-HERC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HERC3 purchase

MBS6206592 is an antibody targeting HERC3. Also known as HERC3 (hect Domain and RLD 3, KIAA0032) (MaxLight 490). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HERC3. It may not necessarily be applicable to this product.

NCBI GI #

7657152

NCBI GeneID

8916

NCBI Accession #

NP_055421

UniProt Primary Accession #

Q15034

UniProt Related Accession #

Q15034

GenBank Nucleotide #

NP_055421.1

NCBI Official Full Name

probable E3 ubiquitin-protein ligase HERC3 isoform 1

NCBI Official Synonym Full Names

HECT and RLD domain containing E3 ubiquitin protein ligase 3

NCBI Official Symbol

HERC3

UniProt Protein Name

Probable E3 ubiquitin-protein ligase HERC3

UniProt Synonym Protein Names

HECT domain and RCC1-like domain-containing protein 3; HECT-type E3 ubiquitin transferase HERC3

Protein Family

Probable E3 ubiquitin-protein ligase

UniProt Gene Name

HERC3

UniProt Synonym Gene Names

KIAA0032

NCBI Protein Information

probable E3 ubiquitin-protein ligase HERC3

NCBI Summary for HERC3

This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]

UniProt Comments for HERC3

HERC3: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol

Biological Process: protein ubiquitination

Research Articles on HERC3

1. The data illuminate a novel axis regulating cell senescence: DeltaNp63alpha stimulates transcription of E3 ligase HERC3, which mediates ubiquitination of c-Myc modulator MM1 and targets it to proteasomal degradation; subsequently, c-Myc is derepressed by DeltaNp63alpha, thereby cell senescence is modulated by this axis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.