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G Protein Gi Alpha1/Alpha2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620758

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene… More details

$980 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
G Protein Gi alpha1/alpha2, Polyclonal Antibody
Full Product Name
G Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2)
Product Synonym Names
Anti -G Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine, Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, 0.08% sodium azide.
Purity/Purification
Purified
Purified
Specificity
Recognizes human G protein subunit alphai1 and alpha2. Species Crossreactivity: mouse, rat, bovine.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa345-354 of Gi alpha1 and 346-355 of Gi alpha2.
Positive Control
L6 Cell Lysate

Product Categories/Family for anti-G Protein Gi alpha1/alpha2 antibody

Antibodies; Abs to G Proteins
Applications Tested/Suitable for anti-G Protein Gi alpha1/alpha2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-G Protein Gi alpha1/alpha2 antibody
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:100

Immunohistochemistry (IHC) of anti-G Protein Gi alpha1/alpha2 antibody

Representative images from a previous lot. Optimal staining of MBS620758 with Citrate pH 6.0 epitope retrieval: rat cerebellum Representative staining pattern/morphology in rat cerebellum using MBS620758. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of MBS620758 was diluted to 1:100, using IHC-Select® Detection with HRP-DAB
Rabbit Anti-G Protein Gi Alpha1/Alpha2 Polyclonal Antibody – Cat# MBS620758

Chemiluminescence of anti-G Protein Gi alpha1/alpha2 antibody

L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620758 (1:500). Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates MBS620758(~40kD).
Rabbit Anti-G Protein Gi Alpha1/Alpha2 Polyclonal Antibody, Chemiluminescence – Cat# MBS620758

Testing Data of anti-G Protein Gi alpha1/alpha2 antibody

Representative image from a previous lot. Huvec lysate (Lane 1), A431 lysate (Lane 2), L6 lysate (Lane 3) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620758 (1:500) Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates G protein Gialpha 1 and 2 (~40kD).
Rabbit Anti-G Protein Gi Alpha1/Alpha2 Polyclonal Antibody, Testing Data – Cat# MBS620758

Other Notes

Small volumes of anti-G Protein Gi alpha1/alpha2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G Protein Gi alpha1/alpha2 purchase

MBS620758 is an antibody targeting G Protein Gi alpha1/alpha2. Also known as G Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2). Reactive in Bovine, Human, Mouse, Rat. Host: Rabbit.
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