TOPBP1 Antibody – Mouse Monoclonal

TOPBP1: a DNA-binding protein and scaffolding protein that is required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. Contains 7 BRCT domains, which are characteristic of proteins involved in cell cycle checkpoint control in response to DNA damage. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase. Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: male germ cell nucleus; spindle pole; Golgi apparatus; centrosome; PML body; condensed nuclear chromosome; cytoplasm; chromosome; nucleus; actin cytoskeleton

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; DNA binding

Biological Process: response to ionizing radiation; DNA repair; DNA metabolic process; response to DNA damage stimulus