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EHMT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS621075

G9a (BAT8 or Histone-lysine N-methyltransferase) is thought to be involved in intracellular protein-protein interaction and is a lysine-preferring histone methyltransferase. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional… More details

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Product Overview

Online Datasheet in PDF
Product Name
G9a (EHMT2), Polyclonal Antibody
Full Product Name
G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/G9a
Product Gene Name
anti-EHMT2 antibody
Product Synonym Names
Anti -G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/G9a
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15mM sodium chloride, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human G9a. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
132,370 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (31847536..31865464, complement). Location: 6p21.31
OMIM
604599
3D Structure
ModBase 3D Structure for Q96KQ7
Immunogen
Linear peptide corresponding to G9a (KLH). Cellular Localization: Nucleus.
Positive Control
HeLa cell lysate

Product Categories/Family for anti-EHMT2 antibody

Antibodies; Abs to Enzymes; Methyltransferase
Applications Tested/Suitable for anti-EHMT2 antibody
Western Blot (WB)
Application Notes for anti-EHMT2 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 0.25-2ug/ml

Western Blot (WB) of anti-EHMT2 antibody

Western Blot Analysis: Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-G9a (0.25ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G9a (~160kD).
Rabbit Anti-EHMT2 Polyclonal Antibody – Cat# MBS621075

Western Blot (WB) of anti-EHMT2 antibody

Western Blot Analysis (SNAP ID Technique): Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with Anti-G9a (1ug/ml). Proteins were visualized using a Snap ID, a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G9a (~160kD).
Rabbit Anti-EHMT2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS621075

Other Notes

Small volumes of anti-EHMT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EHMT2 purchase

MBS621075 is an antibody targeting EHMT2. Also known as G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/G9a. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EHMT2. It may not necessarily be applicable to this product.

NCBI GI #
287865
NCBI GeneID
10919
UniProt Primary Accession #
Q96KQ7
UniProt Secondary Accession #
Q14349; Q5JP83; Q5JQ92; Q5JQA1; Q5JQG3; Q6PK06; Q96MH5; Q96QD0; Q9UQL8; Q9Y331; B0UZY2
UniProt Related Accession #
Q59FM7; Q5JQA2; Q96KQ7
NCBI Official Full Name
G9a
NCBI Official Synonym Full Names
euchromatic histone-lysine N-methyltransferase 2
NCBI Official Symbol
EHMT2
NCBI Official Synonym Symbols
G9A; BAT8; GAT8; NG36; KMT1C; C6orf30; FLJ35547; DKFZp686H08213
UniProt Protein Name
Histone-lysine N-methyltransferase EHMT2
UniProt Synonym Protein Names
Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Lysine N-methyltransferase 1C; Protein G9a
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
EHMT2
UniProt Synonym Gene Names
BAT8; C6orf30; G9A; KMT1C; NG36
UniProt Entry Name
EHMT2_HUMAN
NCBI Protein Information
histone-lysine N-methyltransferase EHMT2; protein G9a; H3-K9-HMTase 3; OTTHUMP00000029262; OTTHUMP00000029264; OTTHUMP00000174666; OTTHUMP00000174667; G9A histone methyltransferase; HLA-B associated transcript 8; HLA-B-associated transcript 8; lysine N-methyltransferase 1C; ankyrin repeat-containing protein; histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase, H3 lysine-9 specific 3

NCBI Summary for EHMT2

A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described. [provided by RefSeq]
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UniProt Comments for EHMT2

EHMT2 iso4: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleus

Molecular Function: protein binding; zinc ion binding; p53 binding; protein-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of DNA replication; histone H3-K9 methylation
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Research Articles on EHMT2

1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EHMT2 antibody

 Reference Product  PubMed Publications
 REST antibody  >1 publications with EHMT2 and REST

Pathways associated with anti-EHMT2 antibody

 Products by Pathway  Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram
 RNA Polymerase I Promoter Clearance Pathway antibodies  RNA Polymerase I Promoter Clearance Pathway Diagram
 RNA Polymerase I Transcription Pathway antibodies  RNA Polymerase I Transcription Pathway Diagram
 RNA Polymerase I Transcription Initiation Pathway antibodies  RNA Polymerase I Transcription Initiation Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 Regulation Of Androgen Receptor Activity Pathway antibodies  Regulation Of Androgen Receptor Activity Pathway Diagram
 Transcription Pathway antibodies  Transcription Pathway Diagram

Diseases associated with anti-EHMT2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with EHMT2 and Neoplasms
 Inflammation Antibodies  >3 publications with EHMT2 and Inflammation
 Necrosis Antibodies  >3 publications with EHMT2 and Necrosis
 Leukemia Antibodies  >2 publications with EHMT2 and Leukemia
 Adenocarcinoma Antibodies  >2 publications with EHMT2 and Adenocarcinoma
 Carcinoma Antibodies  >2 publications with EHMT2 and Carcinoma
 Breast Neoplasms Antibodies  >2 publications with EHMT2 and Breast Neoplasms
 Colorectal Neoplasms Antibodies  >1 publications with EHMT2 and Colorectal Neoplasms
 Neoplasm Metastasis Antibodies  >1 publications with EHMT2 and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >1 publications with EHMT2 and Lung Neoplasms

Organs/Tissues associated with anti-EHMT2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with EHMT2 and Blood
 Liver Antibodies  >3 publications with EHMT2 and Liver
 Placenta Antibodies  >2 publications with EHMT2 and Placenta
 Thyroid Antibodies  >2 publications with EHMT2 and Thyroid
 Lung Antibodies  >2 publications with EHMT2 and Lung
 Bone Antibodies  >1 publications with EHMT2 and Bone
 Stomach Antibodies  >1 publications with EHMT2 and Stomach
 Embryonic Tissue Antibodies  >1 publications with EHMT2 and Embryonic Tissue
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