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CENP-Q (CENPQ) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS621140

Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex… More details

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Product Overview

Online Datasheet in PDF
Product Name
CENP-Q (CENPQ), Polyclonal Antibody
Full Product Name
CENP-Q (Centromere Protein Q)
Product Gene Name
anti-CENPQ antibody
Product Synonym Names
Anti -CENP-Q (Centromere Protein Q)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Species Sequence Homology: macaque(91%), horse(76%), bovine(74%), dog(73%), swine (71%), mouse(65%) and rat(60%).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
~26-31kD
Chromosome Location
Chromosome: 6; NC_000006.11 (49431096..49460820). Location: 6p12.3
OMIM
611506
3D Structure
ModBase 3D Structure for Q7L2Z9
Immunogen
Full-length human CENP-Q recombinant protein.

Product Categories/Family for anti-CENPQ antibody

Antibodies; Abs to Centromeres
Applications Tested/Suitable for anti-CENPQ antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-CENPQ antibody
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 1:100-1:500
ELISA: 1:20,000-1:30,000
Western Blot: 1:2,000-1:2,500

Western Blot (WB) of anti-CENPQ antibody

Western blot using Immunochemicals’ protein A purified anti- CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5_g/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus.
Rabbit Anti-CENPQ Polyclonal Antibody – Cat# MBS621140

Immunofluorescence (IF) of anti-CENPQ antibody

Immunofluorescence microscopy using Immunochemicals’ protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide.
Rabbit Anti-CENPQ Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS621140

Other Notes

Small volumes of anti-CENPQ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CENPQ purchase

MBS621140 is an antibody targeting CENPQ. Also known as CENP-Q (Centromere Protein Q). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CENPQ. It may not necessarily be applicable to this product.

NCBI GI #
40068061
NCBI GeneID
55166
NCBI Accession #
NP_060602.2
UniProt Primary Accession #
Q7L2Z9
UniProt Secondary Accession #
Q6IN61; Q9NVS5; A8KAF1
UniProt Related Accession #
Q7L2Z9
GenBank Nucleotide #
NM_018132.3
NCBI Official Full Name
centromere protein Q
NCBI Official Synonym Full Names
centromere protein Q
NCBI Official Symbol
CENPQ
NCBI Official Synonym Symbols
CENP-Q; C6orf139; FLJ10545
UniProt Protein Name
Centromere protein Q
Protein Family
Centromere protein
UniProt Gene Name
CENPQ
UniProt Synonym Gene Names
C6orf139
UniProt Entry Name
CENPQ_HUMAN
NCBI Protein Information
centromere protein Q; OTTHUMP00000016579

NCBI Summary for CENPQ

CENPQ is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM]

UniProt Comments for CENPQ

CENPQ: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: nucleoplasm; cytosol; chromosome, pericentric region; actin cytoskeleton

Biological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CENPQ antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway antibodies  Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic M-M/G1 Phases Pathway antibodies  Mitotic M-M/G1 Phases Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 Nucleosome Assembly Pathway antibodies  Nucleosome Assembly Pathway Diagram
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