Mouse Anti-CK-19 Monoclonal Antibody – Cat# MBS6214995

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human colon cancer cells stained with 138560 (1:100).

Keratin 19 (CK-19) Antibody – Mouse Monoclonal

Name: CK-19 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6214995
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6214995

MBS6214995 is an antibody targeting CK-19. Also known as Keratin 19 (K19, Keratin-19, CK-19, Cytokeratin-19) (MaxLight 550). Reactive in Human. Host: Mouse. More details

$1105 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Keratin 19 (CK-19), Monoclonal Antibody

Full Product Name

Keratin 19 (K19, Keratin-19, CK-19, Cytokeratin-19) (MaxLight 550)

Product Gene Name

anti-CK-19 antibody

Clonality

Monoclonal

Isotype

IgG2a,k

Clone Number

A53-B/A2.26, Ks 19.1

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

Purity/Purification

Purified by Protein A/G Affinity Chromatography.

Specificity

Recognizes the rod domain of human keratin 19. Epitope: aa312-335. Reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Does not react with rat or ferret.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

44,106 Da

OMIM

148020

3D Structure

ModBase 3D Structure for P08727

Immunogen

Human breast cancer MCF-7 cells. Swissprot: P08727

Conjugate

MaxLight550

Positive Control

MCF-7, HeLa, Hep-G2 cells, Breast Cancer

Product Categories/Family for anti-CK-19 antibody

Antibodies; Keratin; Cytokeratin

Applications Tested/Suitable for anti-CK-19 antibody

Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Application Notes for anti-CK-19 antibody

IHC-P: Formalin fixed paraffin embedded. Requires HIER using sodium citrate buffer pH6.0
Applications are based on unconjugated antibody.

Product References and Citations for anti-CK-19 antibody

1. Bartek J et. al. Histochemical Journal, 1990, 22(10):537-44. 2. Karsten et al., J Cancer Clin. Oncol 21(6) 733-740 (1985)

Other Notes

Small volumes of anti-CK-19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CK-19. It may not necessarily be applicable to this product.

NCBI GI #

24234699

NCBI GeneID

3880

NCBI Accession #

NP_002267

UniProt Primary Accession #

P08727

UniProt Secondary Accession #

Q5XG83; Q6NW33; Q7L5M9; Q96A53; Q96FV1; Q9BYF9; Q9P1Y4; B2R874

UniProt Related Accession #

P08727

GenBank Nucleotide #

NM_002276.4

NCBI Official Full Name

keratin, type I cytoskeletal 19

NCBI Official Synonym Full Names

keratin 19

NCBI Official Symbol

KRT19

NCBI Official Synonym Symbols

K19; CK19; K1CS

UniProt Protein Name

Keratin, type I cytoskeletal 19

UniProt Synonym Protein Names

Cytokeratin-19; CK-19; Keratin-19

Protein Family

Keratin

UniProt Gene Name

KRT19

UniProt Synonym Gene Names

CK-19; K19

UniProt Entry Name

K1C19_HUMAN

NCBI Protein Information

keratin, type I cytoskeletal 19

NCBI Summary for CK-19

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

UniProt Comments for CK-19

K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: dystrophin-associated glycoprotein complex; costamere; intermediate filament; Z disc; sarcolemma

Molecular Function: protein binding; structural constituent of cytoskeleton; structural constituent of muscle; protein complex binding

Biological Process: viral reproduction; response to estrogen stimulus; sarcomere organization

Research Articles on CK-19

1. Our results are of special importance against the background that CK19 positive carcinomas revealed a significantly poorer prognosis and therefore emphasize its prognostic and possible diagnostic role in tongue cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CK-19 antibody

Reference Product	PubMed Publications
KRT15 antibody	>3 publications with CK-19 and KRT15
KRT6B antibody	>1 publications with CK-19 and KRT6B
FANCG antibody	>1 publications with CK-19 and FANCG

Pathways associated with anti-CK-19 antibody

Products by Pathway	Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Keratinization Pathway antibodies	Keratinization Pathway Diagram
Signaling Mediated By P38-alpha And P38-beta Pathway antibodies	Signaling Mediated By P38-alpha And P38-beta Pathway Diagram

Diseases associated with anti-CK-19 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>42 publications with CK-19 and Neoplasms
Carcinoma Antibodies	>25 publications with CK-19 and Carcinoma
Adenocarcinoma Antibodies	>13 publications with CK-19 and Adenocarcinoma
Breast Neoplasms Antibodies	>9 publications with CK-19 and Breast Neoplasms
Liver Diseases Antibodies	>8 publications with CK-19 and Liver Diseases
Neoplasm Metastasis Antibodies	>7 publications with CK-19 and Neoplasm Metastasis
Liver Neoplasms Antibodies	>5 publications with CK-19 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>5 publications with CK-19 and Carcinoma, Hepatocellular
Kidney Diseases Antibodies	>4 publications with CK-19 and Kidney Diseases
Fibrosis Antibodies	>4 publications with CK-19 and Fibrosis

Organs/Tissues associated with anti-CK-19 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>12 publications with CK-19 and Liver
Blood Antibodies	>12 publications with CK-19 and Blood
Lung Antibodies	>6 publications with CK-19 and Lung
Bone Antibodies	>5 publications with CK-19 and Bone
Kidney Antibodies	>4 publications with CK-19 and Kidney
Thyroid Antibodies	>4 publications with CK-19 and Thyroid
Eye Antibodies	>3 publications with CK-19 and Eye
Stomach Antibodies	>3 publications with CK-19 and Stomach
Pancreas Antibodies	>3 publications with CK-19 and Pancreas
Lymph Antibodies	>2 publications with CK-19 and Lymph