Mouse Anti-PMEL Monoclonal Antibody – Cat# MBS6215003

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human melanoma tissue stained with 138569-AF, peroxidase-conjugate and DAB chromogen.

Melanoma (PMEL) Antibody – Mouse Monoclonal

Name: PMEL Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6215003
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6215003

MBS6215003 is an antibody targeting PMEL. Also known as Melanoma, gp100 (pMel17, Melanocyte Protein PMEL, Melanocytes Lineage-specific Antigen GP100) (MaxLight 550). Reactive in Equine, Human. Host: Mouse. More details

$1105 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Melanoma (PMEL), Monoclonal Antibody

Full Product Name

Melanoma, gp100 (pMel17, Melanocyte Protein PMEL, Melanocytes Lineage-specific Antigen GP100) (MaxLight 550)

Product Gene Name

anti-PMEL antibody

Clonality

Monoclonal

Isotype

IgG2b,k

Clone Number

NKI-beteb

Host

Mouse

Species Reactivity

Equine, Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

Purity/Purification

Purified by Protein A/G Affinity Chromatography.

Specificity

Recognizes human Melanoma. Species Crossreactivity: equine. Does not react with rat.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

90100

OMIM

155550

3D Structure

ModBase 3D Structure for P40967

Immunogen

Membranes from a human melanoma metastasis. Uniprot: P40967

Conjugate

MaxLight550

Positive Control

SK-MEL-28 cells or Melanoma

Product Categories/Family for anti-PMEL antibody

Antibodies; Cancer Markers

Applications Tested/Suitable for anti-PMEL antibody

Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin

Application Notes for anti-PMEL antibody

IHC: Formalin-fixed, paraffin-embedded sections. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Applications are based on unconjugated antibody.

Product References and Citations for anti-PMEL antibody

1. Leonhardt RM et al. Proprotein convertases process Pmel17 during secretion. J Biol Chem 286:9321-37 (2011). Flow Cyt ; Human. 2. Leonhardt RM et al. Endoplasmic reticulum export, subcellular distribution, and fibril formation by Pmel17 require an intact N-terminal domain junction. J Biol Chem 285:16166-83 (2010).

Other Notes

Small volumes of anti-PMEL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PMEL. It may not necessarily be applicable to this product.

NCBI GI #

318037593

NCBI GeneID

6490

NCBI Accession #

NP_001186982

UniProt Primary Accession #

P40967

UniProt Related Accession #

P40967

GenBank Nucleotide #

NP_001186982.1

NCBI Official Full Name

melanocyte protein PMEL isoform 2

NCBI Official Synonym Full Names

premelanosome protein

NCBI Official Symbol

PMEL

NCBI Official Synonym Symbols

P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E

UniProt Protein Name

Melanocyte protein PMEL

UniProt Synonym Protein Names

ME20-M; ME20M; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; P1; P100; Premelanosome protein; Silver locus protein homologCleaved into the following 2 chains:M-alpha; Alternative name(s):; 95 kDa melanocyte-specific secreted glycoprotein; P26; Secreted melanoma-associated ME20 antigen

Protein Family

Melanocyte protein

UniProt Gene Name

PMEL

UniProt Synonym Gene Names

D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S

UniProt Entry Name

PMEL_HUMAN

NCBI Protein Information

melanocyte protein PMEL

NCBI Summary for PMEL

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

UniProt Comments for PMEL

SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; plasma membrane; melanosome; integral to membrane; extracellular region

Molecular Function: protein binding

Biological Process: melanin biosynthetic process; melanosome organization and biogenesis

Research Articles on PMEL

1. pH-Dependent fibril maturation of a Pmel17 repeat domain isoform revealed by tryptophan fluorescence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-PMEL antibody

Disease Name	Pubmed Publications
Skin Diseases Antibodies	>115 publications with PMEL and Skin Diseases
Disease Models, Animal Antibodies	>81 publications with PMEL and Disease Models, Animal
Necrosis Antibodies	>17 publications with PMEL and Necrosis
Lung Diseases Antibodies	>15 publications with PMEL and Lung Diseases
Brain Diseases Antibodies	>9 publications with PMEL and Brain Diseases
Leukopenia Antibodies	>6 publications with PMEL and Leukopenia
Neutropenia Antibodies	>6 publications with PMEL and Neutropenia
Pain Antibodies	>5 publications with PMEL and Pain
Kidney Diseases Antibodies	>5 publications with PMEL and Kidney Diseases
Drug Hypersensitivity Antibodies	>4 publications with PMEL and Drug Hypersensitivity

Organs/Tissues associated with anti-PMEL antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>127 publications with PMEL and Skin
Lung Antibodies	>22 publications with PMEL and Lung
Eye Antibodies	>21 publications with PMEL and Eye
Spleen Antibodies	>19 publications with PMEL and Spleen
Brain Antibodies	>16 publications with PMEL and Brain
Kidney Antibodies	>12 publications with PMEL and Kidney
Bone Antibodies	>10 publications with PMEL and Bone
Testis Antibodies	>8 publications with PMEL and Testis
Liver Antibodies	>8 publications with PMEL and Liver
Muscle Antibodies	>7 publications with PMEL and Muscle