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IgG, F(ab')2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS621748

MBS621748 is an antibody targeting IgG, F(ab')2. Also known as IgG, F(ab')2 (Biotin). Reactive in Rat. Host: Rabbit. More details

$755 / 2 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG, F(ab')2, Polyclonal Antibody
Full Product Name
IgG, F(ab')2 (Biotin)
Product Synonym Names
Anti -IgG, F(ab')2 (Biotin)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.01% thimerosal.
Purity/Purification
Affinity Purified
99% Purified by Rat IgG-agarose affinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit se
Specificity
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, rat IgG and rat serum and rat IgG F(ab')2. No reaction was observed against rat IgG F(c).
Preparation and Storage
Store vial at 4 degree C prior to restoration. Restore with 1ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Immunogen
Rat IgG F(ab')2 fragment.
Biotin/Protein Ratio
10-20 BAC molecules per rabbit IgG molecule
Applications Tested/Suitable for anti-IgG, F(ab')2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IgG, F(ab')2 antibody
Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy and antibody based assays using streptavidin or avidin conjugates.
Dilution: Assayed against 1ug of Rat IgG in a standard capture ELISA using peroxidase conjugated streptavidin. A working dilution of 1:6,000 to 1:25,000 of the reconstitution concentration is suggested.

Other Notes

Small volumes of anti-IgG, F(ab')2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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