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ARFGAP3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS621919

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which is thought to interact with ADP-ribosylation factor 1 (ARF1). The encoded protein likely promotes hydrolysis of ARF1-bound GTP, which is… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ARFGAP3, Polyclonal Antibody
Full Product Name
ARFGAP3 (ADP-ribosylation factor GTPase activating protein 3, ARFGAP1, RP1-47A17.3, ADP-ribosylation factor GTPase activating protein 1, OTTHUMP00000028526)
Product Gene Name
Product Synonym Names
Anti -ARFGAP3 (ADP-ribosylation factor GTPase activating protein 3, ARFGAP1, RP1-47A17.3, ADP-ribosylation factor GTPase activating protein 1, OTTHUMP00000028526)
Clonality
Polyclonal
Host
Goat
Species Reactivity
Canine, Human, Mouse, Rabbit
Form/Format
Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes ARFGAP3 at ~55-60kD in mouse brain extracts. Species Crossreactivity: Canine, mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
56,928 Da
Chromosome Location
Chromosome: 22; NC_000022.10 (43192532..43253408, complement). Location: 22q13.2
OMIM
612439
3D Structure
ModBase 3D Structure for Q9NP61
Immunogen
Synthetic peptide of ARFGAP3.
Applications Tested/Suitable for anti-ARFGAP3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-ARFGAP3 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1-3ug/ml

Testing Data of anti-ARFGAP3 antibody

blot analysis of ARFGAP3 in mouse brain extracts (RIPA buffer, 35μg total protein per lane) using ARFGAP3 antibody (2μg/ml).
Goat Anti-ARFGAP3 Polyclonal Antibody – Cat# MBS621919

Other Notes

Small volumes of anti-ARFGAP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARFGAP3 purchase

MBS621919 is an antibody targeting ARFGAP3. Also known as ARFGAP3 (ADP-ribosylation factor GTPase activating protein 3, ARFGAP1, RP1-47A17.3, ADP-ribosylation factor GTPase activating protein 1, OTTHUMP00000028526). Reactive in Canine, Human, Mouse, Rabbit. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for ARFGAP3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ADP-ribosylation factor GTPase-activating protein 3 isoform 1
NCBI Official Synonym Full Names
ADP-ribosylation factor GTPase activating protein 3
NCBI Official Symbol
ARFGAP3
NCBI Official Synonym Symbols
ARFGAP1; FLJ45618
UniProt Protein Name
ADP-ribosylation factor GTPase-activating protein 3
UniProt Gene Name
ARFGAP3
UniProt Synonym Gene Names
ARFGAP1
UniProt Entry Name
ARFG3_HUMAN
NCBI Protein Information
ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; OTTHUMP00000198439; OTTHUMP00000221483; ADP-ribosylation factor GTPase activating protein 1

NCBI Summary for ARFGAP3

The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq]
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UniProt Comments for ARFGAP3

ARF GAP 3: GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.

Protein type: GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: Golgi membrane; Golgi apparatus; membrane; cytoplasm; cytosol

Molecular Function: protein binding; zinc ion binding; protein transporter activity

Biological Process: vesicle-mediated transport; intracellular protein transport; protein secretion; positive regulation of GTPase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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