Mouse Anti-ATG5 Monoclonal Antibody – Cat# MBS6220563

ATG5 Antibody – Mouse Monoclonal

Name: ATG5 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6220563
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6220563

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. APG5 conjugates to APG12 and… More details

$1060 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ATG5, Monoclonal Antibody

Full Product Name

ATG5 (Autophagy Protein 5, APG5-like, Apoptosis-specific Protein, APG5L, ASP) (MaxLight 650)

Product Gene Name

anti-ATG5 antibody

Clonality

Monoclonal

Isotype

IgG2a,k

Clone Number

22CT1.11.4.4

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

Purity/Purification

Purified by Protein G Affinity Chromatography

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

275

OMIM

604261

3D Structure

ModBase 3D Structure for Q9H1Y0

Immunogen

Purified recombinant APG5 fusion protein.

Conjugate

MaxLight650

Product Categories/Family for anti-ATG5 antibody

Antibodies; Autophagy

Applications Tested/Suitable for anti-ATG5 antibody

FLISA, Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-ATG5 antibody

FLISA: 1:1000
IHC: 1:50-100
WB: 1:1000
Applications are based on unconjugated antibody.

Product References and Citations for anti-ATG5 antibody

Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744. Autophagy protein ATG5 interacts transiently with the hepatitis C virus RNA polymerase (NS5B) early during infection. Guvin C, et al. Virology, 2010 Sep 15. PMID 20580051. The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells. Gao P, et al. J Biol Chem, 2010 Aug 13. PMID 20529838. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614. Morphine induces Beclin 1- and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus. Zhao L, et al. Autophagy, 2010 Apr. PMID 20190558.

Other Notes

Small volumes of anti-ATG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG5 purchase

MBS6220563 is an antibody targeting ATG5. Also known as ATG5 (Autophagy Protein 5, APG5-like, Apoptosis-specific Protein, APG5L, ASP) (MaxLight 650). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG5. It may not necessarily be applicable to this product.

NCBI GI #

17366828

NCBI GeneID

9474

NCBI Accession #

Q9H1Y0.2

UniProt Primary Accession #

Q9H1Y0

UniProt Secondary Accession #

O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7

UniProt Related Accession #

Q9H1Y0

NCBI Official Full Name

Autophagy protein 5

NCBI Official Synonym Full Names

autophagy related 5

NCBI Official Symbol

ATG5

NCBI Official Synonym Symbols

ASP; APG5; APG5L; hAPG5; APG5-LIKE

UniProt Protein Name

Autophagy protein 5

UniProt Synonym Protein Names

APG5-like; Apoptosis-specific protein

Protein Family

Autophagy protein

UniProt Gene Name

ATG5

UniProt Synonym Gene Names

APG5L; ASP

UniProt Entry Name

ATG5_HUMAN

NCBI Protein Information

autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog

UniProt Comments for ATG5

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy; Apoptosis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; autophagy; innate immune response; negative regulation of protein ubiquitination; blood vessel remodeling; negative regulation of interferon type I production; otolith development; autophagic vacuole formation; negative regulation of apoptosis

Research Articles on ATG5

1. Globular adiponectin restores ethanol-suppressed autophagy-related protein 5 (Atg5) expression in HepG2 cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG5 antibody

Reference Product	PubMed Publications
ATG12 antibody	>223 publications with ATG5 and ATG12
BECN1 antibody	>194 publications with ATG5 and BECN1
ATG3 antibody	>53 publications with ATG5 and ATG3
ATG16L1 antibody	>48 publications with ATG5 and ATG16L1
CASP8 antibody	>27 publications with ATG5 and CASP8
FADD antibody	>21 publications with ATG5 and FADD
SIRT1 antibody	>8 publications with ATG5 and SIRT1
DDX58 antibody	>5 publications with ATG5 and DDX58
MAVS antibody	>4 publications with ATG5 and MAVS
WDFY3 antibody	>1 publications with ATG5 and WDFY3

Pathways associated with anti-ATG5 antibody

Products by Pathway	Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies	Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
RIG-I-like Receptor Signaling Pathway antibodies	RIG-I-like Receptor Signaling Pathway Diagram
RIG-I-like Receptor Signaling Pathway antibodies	RIG-I-like Receptor Signaling Pathway Diagram
RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways antibodies	RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways Diagram
Regulation Of Autophagy Pathway antibodies	Regulation Of Autophagy Pathway Diagram
Regulation Of Autophagy Pathway antibodies	Regulation Of Autophagy Pathway Diagram
Senescence And Autophagy Pathway antibodies	Senescence And Autophagy Pathway Diagram
Shigellosis Pathway antibodies	Shigellosis Pathway Diagram

Diseases associated with anti-ATG5 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>57 publications with ATG5 and Necrosis
Cardiovascular Diseases Antibodies	>55 publications with ATG5 and Cardiovascular Diseases
Inflammation Antibodies	>55 publications with ATG5 and Inflammation
Disease Models, Animal Antibodies	>47 publications with ATG5 and Disease Models, Animal
Liver Diseases Antibodies	>42 publications with ATG5 and Liver Diseases
Gastrointestinal Diseases Antibodies	>35 publications with ATG5 and Gastrointestinal Diseases
Heart Diseases Antibodies	>34 publications with ATG5 and Heart Diseases
Carcinoma, Hepatocellular Antibodies	>27 publications with ATG5 and Carcinoma, Hepatocellular
Breast Neoplasms Antibodies	>25 publications with ATG5 and Breast Neoplasms
Liver Neoplasms Antibodies	>24 publications with ATG5 and Liver Neoplasms

Organs/Tissues associated with anti-ATG5 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>91 publications with ATG5 and Blood
Brain Antibodies	>88 publications with ATG5 and Brain
Liver Antibodies	>69 publications with ATG5 and Liver
Muscle Antibodies	>59 publications with ATG5 and Muscle
Lung Antibodies	>58 publications with ATG5 and Lung
Heart Antibodies	>52 publications with ATG5 and Heart
Kidney Antibodies	>35 publications with ATG5 and Kidney
Eye Antibodies	>31 publications with ATG5 and Eye
Bone Antibodies	>31 publications with ATG5 and Bone
Prostate Antibodies	>25 publications with ATG5 and Prostate