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Rsk, 90kD (RPS6KA1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS622410

The 90kD ribosomal S6 kinases (RSK1-3) are a family of broadly expressed serine/ threonine kinases activated by extracellular signal-regulated protein kinases (Erk1 and 2) in response to many growth factors, polypeptide hormones and neurotransmitters. p90RSK… More details

$830 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Rsk, 90kD (RPS6KA1), Polyclonal Antibody
Full Product Name
Rsk, 90kD, phosphorylated (Thr573) (Ribosomal S6 Kinase, p90RSK)
Product Gene Name
anti-RPS6KA1 antibody
Product Synonym Names
Anti -Rsk, 90kD, phosphorylated (Thr573) (Ribosomal S6 Kinase, p90RSK)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Chicken, Hamster, Human, Mouse, Rat, Xenopus
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Also available without BSA. See R9399-01C1.
Purity/Purification
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Specificity
Detects endogenous human p90RSK protein only when phosphorylated at Thr573. Species Crossreactivity: Hamster, mouse and rat. Predicted to crossreact with chicken and Xenopus based on sequence homology.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
82,723 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (26856249..26901520). Location: 1p
OMIM
601684
3D Structure
ModBase 3D Structure for Q15418
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr573 of human p90RSK (90kD) (KLH coupled).

Product Categories/Family for anti-RPS6KA1 antibody

Antibodies; Abs to Protein Kinases; Ribosomal
Applications Tested/Suitable for anti-RPS6KA1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-RPS6KA1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000

Western Blot (WB) of anti-RPS6KA1 antibody

Western Blot analysis of extracts from HeLa, PC12 and NIH/3T3 cells, untreated or TPA-treated, using MBS622410.
Rabbit Anti-RPS6KA1 Polyclonal Antibody – Cat# MBS622410

Immunohistochemistry (IHC) of anti-RPS6KA1 antibody

Confocal images of immunohistochemical staining with MBS622410 (green) and propidium iodide (red) in rat dentate gyrus regions, using sections from control (left) and 15 minute transient cerebral ischemia followed by 30 minutes of reperfusion (right). (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida.)
Rabbit Anti-RPS6KA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS622410

Other Notes

Small volumes of anti-RPS6KA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPS6KA1 purchase

MBS622410 is an antibody targeting RPS6KA1. Also known as Rsk, 90kD, phosphorylated (Thr573) (Ribosomal S6 Kinase, p90RSK). Reactive in Chicken, Hamster, Human, Mouse, Rat, Xenopus. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPS6KA1. It may not necessarily be applicable to this product.

NCBI GI #
55743134
NCBI GeneID
6195
NCBI Accession #
NP_001006666.1
UniProt Primary Accession #
Q15418
UniProt Secondary Accession #
Q5SVM5; Q5SVM8; Q5SVM9; Q96C05; Q9BQK2; A6NGG4; A8K9K7; B2RDY8; B7Z5J0
UniProt Related Accession #
Q15418
GenBank Nucleotide #
NM_001006665.1
NCBI Official Full Name
ribosomal protein S6 kinase alpha-1 isoform b
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 90kDa, polypeptide 1
NCBI Official Symbol
RPS6KA1
NCBI Official Synonym Symbols
RSK; HU-1; RSK1; MAPKAPK1A
UniProt Protein Name
Ribosomal protein S6 kinase alpha-1
UniProt Synonym Protein Names
90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1
Protein Family
Ribosomal protein S6 kinase
UniProt Gene Name
RPS6KA1
UniProt Synonym Gene Names
MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
UniProt Entry Name
KS6A1_HUMAN
NCBI Protein Information
ribosomal protein S6 kinase alpha-1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)

NCBI Summary for RPS6KA1

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for RPS6KA1

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Translation; AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 1p

Cellular Component: nucleoplasm; cytoplasm; spindle; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; magnesium ion binding; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: regulation of transcription in response to stress; axon guidance; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; regulation of translation in response to stress; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; negative regulation of caspase activity; signal transduction; cell cycle; positive regulation of cell growth; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; synaptic transmission; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis
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Research Articles on RPS6KA1

1. results indicate that RSK directly phosphorylates Gab2 on 3 serine residues; findings show RSK-mediated Gab2 phosphorylation inhibits Shp2 recruitment, suggesting RSK mediates a negative-feedback loop that attenuates Gab2-dependent functions including cell motility

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPS6KA1 antibody

 Reference Product  PubMed Publications
 MAPK1 antibody  >29 publications with RPS6KA1 and MAPK1
 MAPK3 antibody  >27 publications with RPS6KA1 and MAPK3
 RPS6 antibody  >6 publications with RPS6KA1 and RPS6
 TSC2 antibody  >6 publications with RPS6KA1 and TSC2
 EIF4B antibody  >5 publications with RPS6KA1 and EIF4B
 TSC1 antibody  >4 publications with RPS6KA1 and TSC1
 CREB1 antibody  >3 publications with RPS6KA1 and CREB1

Pathways associated with anti-RPS6KA1 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CREB Phosphorylation Pathway antibodies  CREB Phosphorylation Pathway Diagram
 CREB Phosphorylation Through The Activation Of Ras Pathway antibodies  CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
 Cytoplasmic Ribosomal Proteins Pathway antibodies  Cytoplasmic Ribosomal Proteins Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram

Diseases associated with anti-RPS6KA1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >33 publications with RPS6KA1 and Neoplasms
 Inflammation Antibodies  >13 publications with RPS6KA1 and Inflammation
 Hypertrophy Antibodies  >11 publications with RPS6KA1 and Hypertrophy
 Disease Models, Animal Antibodies  >10 publications with RPS6KA1 and Disease Models, Animal
 Liver Diseases Antibodies  >10 publications with RPS6KA1 and Liver Diseases
 Cardiovascular Diseases Antibodies  >7 publications with RPS6KA1 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >7 publications with RPS6KA1 and Breast Neoplasms
 Hyperplasia Antibodies  >6 publications with RPS6KA1 and Hyperplasia
 Adenocarcinoma Antibodies  >5 publications with RPS6KA1 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >5 publications with RPS6KA1 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-RPS6KA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >45 publications with RPS6KA1 and Muscle
 Blood Antibodies  >26 publications with RPS6KA1 and Blood
 Brain Antibodies  >24 publications with RPS6KA1 and Brain
 Liver Antibodies  >23 publications with RPS6KA1 and Liver
 Heart Antibodies  >19 publications with RPS6KA1 and Heart
 Lung Antibodies  >8 publications with RPS6KA1 and Lung
 Connective Tissue Antibodies  >7 publications with RPS6KA1 and Connective Tissue
 Vascular Antibodies  >7 publications with RPS6KA1 and Vascular
 Embryonic Tissue Antibodies  >5 publications with RPS6KA1 and Embryonic Tissue
 Bone Antibodies  >5 publications with RPS6KA1 and Bone
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