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IgG, F(ab')2, X-Adsorbed Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS622522

MBS622522 is an antibody targeting IgG, F(ab')2, X-Adsorbed. Also known as IgG, F(ab')2, X-Adsorbed (AP). Reactive in Mouse. Host: Rabbit. More details

$900 / 1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG, F(ab')2, X-Adsorbed, Polyclonal Antibody
Full Product Name
IgG, F(ab')2, X-Adsorbed (AP)
Product Synonym Names
Anti -IgG, F(ab')2, X-Adsorbed (AP)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse IgG, F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, mouse IgG, mouse IgG F(ab')2 and mouse serum. No reactivity was observed against Mouse IgG F(c) or human serum proteins.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.
Immunogen
Mouse IgG F(ab')2 fragment
Applications Tested/Suitable for anti-IgG, F(ab')2, X-Adsorbed antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:1500-1:10,000. Assayed against 1ug of mouse IgG in a standard ELISA using NPP as a substrate for 30 minutes at RT.
Western Blot: 1:500- 1:2500
Immunohistochemistry: 1:200- 1:1000

Other Notes

Small volumes of anti-IgG, F(ab')2, X-Adsorbed antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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