C14ORF169 Antibody – Goat Polyclonal

NO66: Histone demethylase that specifically demethylates 'Lys- 4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation. Belongs to the MINA53/NO66 family. NO66 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.27; Nucleolus

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: histone demethylase activity (H3-K4 specific); protein binding; iron ion binding; histone demethylase activity (H3-K36 specific); oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors

Biological Process: transcription, DNA-dependent; negative regulation of osteoblast differentiation; negative regulation of transcription, DNA-dependent