Sheep Anti-PEA15 Polyclonal Antibody – Cat# MBS622921

Detection of PEA-15. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml sheep anti-PEA-15.

PEA-15 (PEA15) Antibody – Sheep Polyclonal

Name: PEA15 Antibody; Polyclonal; Sheep; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS622921
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS622921

PEA-15 (Phosphoprotein enriched in astrocytes of 15kD) is a 15kD cytoplasmic protein that regulates cell proliferation and apoptosis. It is expressed in multiple cell types such as astrocytes; skeletal muscle and adipocytes. Human PEA-15 is 130aa in length.… More details

$1105 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PEA-15 (PEA15), Polyclonal Antibody

Full Product Name

PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H, PED, Phosphoprotein enriched in diabetes)

Product Gene Name

anti-PEA15 antibody

Product Synonym Names

Anti -PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H, PED, Phosphoprotein enriched in diabetes)

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Species Reactivity

Human, Mouse

Form/Format

Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS. 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human and mouse PEA-15.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

15,040 Da

Chromosome Location

chromosome: 1; Location: 1q21.1

OMIM

603434

3D Structure

ModBase 3D Structure for Q15121

Immunogen

Recombinant human PEA-15 aa 2-130; Q15121 (E. Coli).

Product Categories/Family for anti-PEA15 antibody

Antibodies; Abs to Apoptosis

Applications Tested/Suitable for anti-PEA15 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-PEA15 antibody

Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml

Other Notes

Small volumes of anti-PEA15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PEA15 purchase

MBS622921 is an antibody targeting PEA15. Also known as PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H, PED, Phosphoprotein enriched in diabetes). Reactive in Human, Mouse. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for PEA15. It may not necessarily be applicable to this product.

NCBI GI #

854167

NCBI GeneID

8682

UniProt Primary Accession #

Q15121

UniProt Secondary Accession #

O00511

UniProt Related Accession #

Q14801; Q15121; Q6FHL9; Q96FS5

NCBI Official Full Name

PEA-15

NCBI Official Synonym Full Names

phosphoprotein enriched in astrocytes 15

NCBI Official Symbol

PEA15

NCBI Official Synonym Symbols

PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PEA15

UniProt Protein Name

Astrocytic phosphoprotein PEA-15

UniProt Synonym Protein Names

15 kDa phosphoprotein enriched in astrocytes; Phosphoprotein enriched in diabetes

Protein Family

Astrocytic phosphoprotein

UniProt Gene Name

PEA15

UniProt Entry Name

PEA15_HUMAN

NCBI Protein Information

astrocytic phosphoprotein PEA-15; OTTHUMP00000029887; homolog of mouse MAT-1 oncogene; phosphoprotein enriched in diabetes; Phosphoprotein enriched in astrocytes, 15kD; 15 kDa phosphoprotein enriched in astrocytes

NCBI Summary for PEA15

PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.[supplied by OMIM]

UniProt Comments for PEA15

PEA-15: an ubiquitously expressed anti-apoptotic protein. Inhibits both Fas- and Tnfrsf1a/Tnfr1-mediated caspase-8 activity and apoptosis. Interacts with CASP8/FLICE and FADD. Regulates glucose transport by controlling both the content of GLUT1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of GLUT4 from the cell interior to the surface. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.

Protein type: Motility/polarity/chemotaxis; Apoptosis

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: microtubule associated complex; cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of glucose import; transport; apoptosis; DNA damage checkpoint; carbohydrate transport; response to morphine

Research Articles on PEA15

1. Observational study of gene-disease association. (HuGE Navigator)
2. Results suggest that PEA-15 expression is likely to be associated with the tumorigenesis of alignant pleural mesothelioma.
3. Strategies are being devised to target key signaling events in PED/PEA-15 action aimed at improving glucose tolerance and at facilitating cancer cell death.
4. transfection with PED siRNA, but not with cFLIP siRNA, sensitized NSCLC cells to TRAIL-induced cell death
5. PTEN influences Fas signaling, at least in part, by regulating PEA-15 phosphorylation and activity that, in turn, regulate the ability of Bcl-2 to suppress Fas-induced apoptosis.
6. COUP-TFII antagonizes the repression of the PED/PEA-15 gene by HNF-4alpha.
7. PEA-15 expression is an important prognostic marker in ovarian cancer.
8. Elevated PEA-15 levels provide a survival advantage for glioblastoma cells confronted with a poor microenvironment and low glucose levels.
9. PEA-15 functions as a scaffold to enhance extracellular signal-regulated MAP kinase (ERK) activation of ribosomal S6 kinase polypeptide RSK2, an activity that is regulated by phosphorylation.
10. In addition to sequestering protein kinases ERK1/2 in the cytoplasm, PEA-15 has the potential to modulate the activity of ERK2 in cells by competing directly with proteins that contain D-recruitment sites.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PEA15 antibody

Reference Product	PubMed Publications
CASP8 antibody	>17 publications with PEA15 and CASP8
FADD antibody	>15 publications with PEA15 and FADD
MAPK1 antibody	>11 publications with PEA15 and MAPK1
PLD1 antibody	>4 publications with PEA15 and PLD1
AKT1 antibody	>4 publications with PEA15 and AKT1
PRKCA antibody	>3 publications with PEA15 and PRKCA

Diseases associated with anti-PEA15 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>44 publications with PEA15 and Neoplasms
Insulin Resistance Antibodies	>10 publications with PEA15 and Insulin Resistance
Breast Neoplasms Antibodies	>9 publications with PEA15 and Breast Neoplasms
Skin Diseases Antibodies	>7 publications with PEA15 and Skin Diseases
Lung Neoplasms Antibodies	>5 publications with PEA15 and Lung Neoplasms
Adenocarcinoma Antibodies	>4 publications with PEA15 and Adenocarcinoma
Lung Diseases Antibodies	>4 publications with PEA15 and Lung Diseases
Genital Neoplasms, Female Antibodies	>3 publications with PEA15 and Genital Neoplasms, Female
Skin Neoplasms Antibodies	>3 publications with PEA15 and Skin Neoplasms
Neural Tube Defects Antibodies	>3 publications with PEA15 and Neural Tube Defects

Organs/Tissues associated with anti-PEA15 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>25 publications with PEA15 and Brain
Blood Antibodies	>8 publications with PEA15 and Blood
Muscle Antibodies	>7 publications with PEA15 and Muscle
Ovary Antibodies	>6 publications with PEA15 and Ovary
Lung Antibodies	>6 publications with PEA15 and Lung
Kidney Antibodies	>4 publications with PEA15 and Kidney
Nerve Antibodies	>4 publications with PEA15 and Nerve
Skin Antibodies	>4 publications with PEA15 and Skin
Embryonic Tissue Antibodies	>3 publications with PEA15 and Embryonic Tissue
Thyroid Antibodies	>3 publications with PEA15 and Thyroid