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Nitrotyrosine (NOS2) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS622995

The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Nitrotyrosine (NOS2), Polyclonal Antibody
Full Product Name
Nitrotyrosine (nTyr)
Product Gene Name
anti-NOS2 antibody
Product Synonym Names
Anti -Nitrotyrosine (nTyr)
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity/Purification
Purififed by Ig salt fraction.
Concentration
40 mg/ml (Total protein). (lot specific)
Specificity
Recognizes free Nitrotyrosine and nitrotyrosine-containing proteins.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 19; AC_000176.1 (19758764..19790464, complement). Location: chromosome: 19
3D Structure
ModBase 3D Structure for Q27995
Immunogen
Nitrated KLH
Positive Control
MBS657779: Nitrotyrosine, Immunoblot Positive Control (nTyr).

Product Categories/Family for anti-NOS2 antibody

Antibodies; Abs to Nitrotyrosine
Applications Tested/Suitable for anti-NOS2 antibody
Suitable for use in ELISA (EIA), Western Blot (WB) and Immunohistochemistry (IHC). Other applications not tested.
Application Notes for anti-NOS2 antibody
Western Blot 1:1000
Immunohistochemistry: 1:100-1:10,000

Optimal dilutions to be determined by the researcher.

ELISA (EIA) of anti-NOS2 antibody

Nitrotyrosine antibody specificity. Competition for MBS622995 binding to a NT containing 13mer peptide coated on a plate. ELISA competitors are free nitrotyrosine (diamond), NT containing 33mer peptide (circle), chlorotyrosine (square), and tyrosine (triangle). Competition for Ab binding was most effective with free NT followed closely by the NT containing 33mer peptide. This shows that MBS622995 will recognize free NT as well as NT containing proteins in biological fluids. Significant competition by tyrosine and modified tyrosine occurred at greater than two orders of magnitude higher concentrations of each, indicating very good NT Ab specificity.
Goat Anti-NOS2 Polyclonal Antibody – Cat# MBS622995

Western Blot (WB) of anti-NOS2 antibody

Western Blot using nitrotyrosine immunoblot positive control on nitrocellulose with MBS622995 at 1:1000 dilution. Lane 1, 2.44ug; Lane 2, 9.75ng positive control (nitrated carbonic anhydrase).
Goat Anti-NOS2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS622995

Other Notes

Small volumes of anti-NOS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOS2 purchase

MBS622995 is an antibody targeting NOS2. Also known as Nitrotyrosine (nTyr). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for NOS2. It may not necessarily be applicable to this product.

NCBI GI #
116003827
NCBI GeneID
282876
NCBI Accession #
NP_001070267.1
UniProt Primary Accession #
Q27995
UniProt Secondary Accession #
Q06Q84; Q27985
UniProt Related Accession #
Q27995
GenBank Nucleotide #
NM_001076799.1
NCBI Official Full Name
nitric oxide synthase, inducible
NCBI Official Synonym Full Names
nitric oxide synthase 2, inducible<
NCBI Official Symbol
NOS2
NCBI Official Synonym Symbols
iNOS; NOS2A
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Synonym Protein Names
Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2
Protein Family
Nitric oxide synthase
UniProt Gene Name
NOS2
UniProt Synonym Gene Names
Inducible NOS; iNOS; NOSII
UniProt Entry Name
NOS2_BOVIN
NCBI Protein Information
nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)

UniProt Comments for NOS2

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2

By similarity.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Regulated by calcium/calmodulin

By similarity.

Subunit structure: Homodimer. Binds SLC9A3R1

By similarity.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.
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Research Articles on NOS2

1. Differential cell-specific and spatiotemporal expression of the EDN1 system and NOS in the bovine utero-placental unit may be associated with regulation of vascular and cellular functions during pregnancy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NOS2 antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram

Diseases associated with anti-NOS2 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2258 publications with NOS2 and Inflammation
 Necrosis Antibodies  >1897 publications with NOS2 and Necrosis
 Sepsis Antibodies  >543 publications with NOS2 and Sepsis
 Intestinal Diseases Antibodies  >381 publications with NOS2 and Intestinal Diseases
 Liver Diseases Antibodies  >357 publications with NOS2 and Liver Diseases
 Reperfusion Injury Antibodies  >351 publications with NOS2 and Reperfusion Injury
 Hypertension Antibodies  >283 publications with NOS2 and Hypertension
 Endotoxemia Antibodies  >248 publications with NOS2 and Endotoxemia
 Shock, Septic Antibodies  >236 publications with NOS2 and Shock, Septic
 Kidney Diseases Antibodies  >214 publications with NOS2 and Kidney Diseases

Organs/Tissues associated with anti-NOS2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1149 publications with NOS2 and Muscle
 Intestine Antibodies  >459 publications with NOS2 and Intestine
 Ovary Antibodies  >47 publications with NOS2 and Ovary
 Extraembryonic Tissue Antibodies  >5 publications with NOS2 and Extraembryonic Tissue
 Uncharacterized Tissue Antibodies  >1 publications with NOS2 and Uncharacterized Tissue
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