Sheep Anti-HMGA1 Polyclonal Antibody – Cat# MBS623006

Detection of HMGA1B with MBS623006. 30ug of whole cell extracts from K562, 293T, HeLa and NIH3T3 cells were prepared, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with 1ug/ml sheep anti-HMGA1B antibody.

HMGA1B (HMGA1) Antibody – Sheep Polyclonal

Name: HMGA1 Antibody; Polyclonal; Sheep; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS623006
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS623006

HMGA1B (High mobility group A1, isoform B; also HMG-Y) is a 10-11kD member of the HMGA family of proteins. It is ubiquitously expressed, and occurs principally in tumor and embryonic tissue. HMGA1B is a nonhistone architectural protein that binds to AT-rich… More details

$1105 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HMGA1B, Polyclonal Antibody

Full Product Name

HMGA1B (High mobility group A1, Isoform B, HMG-Y)

Product Gene Name

anti-Hmga1 antibody

Product Synonym Names

Anti -HMGA1B (High mobility group A1, Isoform B, HMG-Y)

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Species Reactivity

Human, Mouse

Form/Format

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS, 0.02% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human HMGA1B at ~20kD. Species Crossreactivity: mouse.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

11,614 Da

Chromosome Location

Chromosome: 17; NC_000083.6 (27556574..27563672). Location: 17 A3.3; 17 14.5 cM

3D Structure

ModBase 3D Structure for P17095

Immunogen

E. coli-derived recombinant human HMGA1B (aa1-96; Accession # P17096).

Product Categories/Family for anti-Hmga1 antibody

Antibodies; Abs to Binding Proteins

Applications Tested/Suitable for anti-Hmga1 antibody

Western Blot (WB)

Application Notes for anti-Hmga1 antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 1ug/ml

Other Notes

Small volumes of anti-Hmga1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hmga1 purchase

MBS623006 is an antibody targeting Hmga1. Also known as HMGA1B (High mobility group A1, Isoform B, HMG-Y). Reactive in Human, Mouse. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for Hmga1. It may not necessarily be applicable to this product.

NCBI GI #

262331580

NCBI GeneID

15361

NCBI Accession #

NP_001160009.1

UniProt Primary Accession #

P17095

UniProt Secondary Accession #

Q91WV2; Q924L7; Q924L8

UniProt Related Accession #

P17095

GenBank Nucleotide #

NM_001166537.1

NCBI Official Full Name

high mobility group protein HMG-I/HMG-Y isoform b

NCBI Official Synonym Full Names

high mobility group AT-hook 1

NCBI Official Symbol

Hmga1

NCBI Official Synonym Symbols

Hmgi; Hmgy; Hmgiy; Hmga1a; Hmga1b; AL023995

UniProt Protein Name

High mobility group protein HMG-I/HMG-Y

UniProt Synonym Protein Names

High mobility group AT-hook protein 1

Protein Family

High mobility group protein

UniProt Gene Name

Hmga1

UniProt Synonym Gene Names

Hmgi; Hmgiy; HMG-I(Y)

UniProt Entry Name

HMGA1_MOUSE

NCBI Protein Information

high mobility group protein HMG-I/HMG-Y; high mobility group protein I; high mobility group protein A1; high mobility group AT-hook protein 1

NCBI Summary for Hmga1

This locus encodes a member of the nuclear, non-histone high mobility group protein family. This architectural transcription factor binds to A-T rich DNA sequences and participates in enhanceosome formation, chromatin remodeling and regulation of transcription. This protein functions in many cellular processes, including cell growth and differentiation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

UniProt Comments for Hmga1

HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: focal adhesion; nucleus

Molecular Function: retinoid X receptor binding; peroxisome proliferator activated receptor binding; enzyme binding; DNA-(apurinic or apyrimidinic site) lyase activity; ligand-dependent nuclear receptor transcription coactivator activity; retinoic acid receptor binding; 5'-deoxyribose-5-phosphate lyase activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; base-excision repair; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; DNA catabolic process, endonucleolytic

Research Articles on Hmga1

1. Increased HMGA1 expression is associated with pituitary and thyroid tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hmga1 antibody

Reference Product	PubMed Publications
Hipk2 antibody	>7 publications with Hmga1 and Hipk2
Ptgs2 antibody	>6 publications with Hmga1 and Ptgs2
Stat3 antibody	>6 publications with Hmga1 and Stat3
Sars antibody	>5 publications with Hmga1 and Sars
Prmt6 antibody	>4 publications with Hmga1 and Prmt6
Crebbp antibody	>3 publications with Hmga1 and Crebbp
Ifng antibody	>1 publications with Hmga1 and Ifng
Srf antibody	>1 publications with Hmga1 and Srf

Pathways associated with anti-Hmga1 antibody

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
IL-4 Signaling Pathway antibodies	IL-4 Signaling Pathway Diagram

Organs/Tissues associated with anti-Hmga1 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>41 publications with Hmga1 and Lung
Muscle Antibodies	>34 publications with Hmga1 and Muscle
Embryonic Tissue Antibodies	>32 publications with Hmga1 and Embryonic Tissue
Liver Antibodies	>28 publications with Hmga1 and Liver
Brain Antibodies	>19 publications with Hmga1 and Brain
Adipose Tissue Antibodies	>15 publications with Hmga1 and Adipose Tissue
Heart Antibodies	>13 publications with Hmga1 and Heart
Connective Tissue Antibodies	>11 publications with Hmga1 and Connective Tissue
Eye Antibodies	>11 publications with Hmga1 and Eye
Thymus Antibodies	>9 publications with Hmga1 and Thymus