Sheep Anti-PIAS2 Polyclonal Antibody – Cat# MBS623132

30ug of whole cell extracts from exponentially growing MCF7, HepG2, HeLa, and Hek293 cells were prepared, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with MBS623132 (0.1ug/m), as described in and incubate the membrane for 1 hour at room temperature in Blocking Solution.

PIAS2 Antibody – Sheep Polyclonal

Name: PIAS2 Antibody; Polyclonal; Sheep; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS623132
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS623132

PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx-) is a 45-70kD member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it… More details

$1105 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PIAS2, Polyclonal Antibody

Full Product Name

PIAS2 (Protein inhibitor of activated STAT, 2 Androgen, receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, MGC102682, miz, Miz1, MIZ1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, PIASX-ALPH

Product Gene Name

anti-PIAS2 antibody

Product Synonym Names

Anti -PIAS2 (Protein inhibitor of activated STAT, 2 Androgen, receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, MGC102682, miz, Miz1, MIZ1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, PIASX-AL

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 100ul sterile 40-50% glycerol, PBS, 0.02% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Detects endogenous human PIAS2 in Western blot with an approximate molecular weight of 63-70kD.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

56,391 Da

Chromosome Location

Chromosome: 18; NC_000018.9 (44392060..44497466, complement). Location: 18q21.1

3D Structure

ModBase 3D Structure for Q2TA77

Immunogen

Purified, E. coli-derived recombinant human PIAS2 aa 1-550; Accession# O75928.

Product Categories/Family for anti-PIAS2 antibody

Antibodies; Abs to Transcription Factors; STAT

Applications Tested/Suitable for anti-PIAS2 antibody

Western Blot (WB)

Application Notes for anti-PIAS2 antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 0.1ug/ml

Other Notes

Small volumes of anti-PIAS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIAS2 purchase

MBS623132 is an antibody targeting PIAS2. Also known as PIAS2 (Protein inhibitor of activated STAT, 2 Androgen, receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, MGC102682, miz, Miz1, MIZ1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, PIASX-ALPH. Reactive in Human. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for PIAS2. It may not necessarily be applicable to this product.

NCBI GI #

83405047

NCBI GeneID

9063

UniProt Primary Accession #

Q2TA77

UniProt Related Accession #

O75928; Q2TA77

NCBI Official Full Name

PIAS2 protein

NCBI Official Synonym Full Names

protein inhibitor of activated STAT, 2

NCBI Official Symbol

PIAS2

NCBI Official Synonym Symbols

miz; MIZ1; SIZ2; PIASX; ZMIZ4; MGC102682; PIASX-BETA; PIASX-ALPHA

UniProt Protein Name

PIAS2 protein

Protein Family

E3 SUMO-protein ligase

UniProt Gene Name

PIAS2

UniProt Entry Name

Q2TA77_HUMAN

NCBI Protein Information

E3 SUMO-protein ligase PIAS2; DIP; ARIP3; PIAS-NY protein; DAB2-interacting protein; Msx-interacting-zinc finger; zinc finger, MIZ-type containing 4; msx-interacting zinc finger protein; protein inhibitor of activated STAT x; androgen receptor-interacting protein 3

NCBI Summary for PIAS2

This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]

UniProt Comments for PIAS2

Sequence similarities: Contains 1 SAP domain. SAAS SAAS003034

Research Articles on PIAS2

1. These findings suggest that SUMO-1 modification of MDA5 possibly via PIAS2beta may play a role in the MDA5-mediated interferon response to viral infections.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PIAS2 antibody

Reference Product	PubMed Publications
PIAS1 antibody	>6 publications with PIAS2 and PIAS1
SUMO1 antibody	>4 publications with PIAS2 and SUMO1
ELK1 antibody	>2 publications with PIAS2 and ELK1
CDKN1A antibody	>1 publications with PIAS2 and CDKN1A
STAT4 antibody	>1 publications with PIAS2 and STAT4
SMAD4 antibody	>1 publications with PIAS2 and SMAD4

Pathways associated with anti-PIAS2 antibody

Products by Pathway	Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Hepatitis C Pathway antibodies	Hepatitis C Pathway Diagram
Hepatitis C Pathway antibodies	Hepatitis C Pathway Diagram
IL12 Signaling Mediated By STAT4 Pathway antibodies	IL12 Signaling Mediated By STAT4 Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Pathways In Cancer antibodies	Pathways In Cancer Diagram
Small Cell Lung Cancer Pathway antibodies	Small Cell Lung Cancer Pathway Diagram
Small Cell Lung Cancer Pathway antibodies	Small Cell Lung Cancer Pathway Diagram
Sumoylation By RanBP2 Regulates Transcriptional Repression Pathway antibodies	Sumoylation By RanBP2 Regulates Transcriptional Repression Pathway Diagram

Diseases associated with anti-PIAS2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>4 publications with PIAS2 and Neoplasms
Nervous System Diseases Antibodies	>2 publications with PIAS2 and Nervous System Diseases
Nerve Degeneration Antibodies	>1 publications with PIAS2 and Nerve Degeneration
Melanoma Antibodies	>1 publications with PIAS2 and Melanoma
Brain Diseases Antibodies	>1 publications with PIAS2 and Brain Diseases
Fibrosis Antibodies	>1 publications with PIAS2 and Fibrosis
Prostatic Neoplasms Antibodies	>1 publications with PIAS2 and Prostatic Neoplasms
Drug Toxicity Antibodies	>1 publications with PIAS2 and Drug Toxicity
Prostatic Diseases Antibodies	>1 publications with PIAS2 and Prostatic Diseases

Organs/Tissues associated with anti-PIAS2 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>2 publications with PIAS2 and Bone
Brain Antibodies	>2 publications with PIAS2 and Brain
Pancreas Antibodies	>1 publications with PIAS2 and Pancreas
Liver Antibodies	>1 publications with PIAS2 and Liver
Embryonic Tissue Antibodies	>1 publications with PIAS2 and Embryonic Tissue
Testis Antibodies	>1 publications with PIAS2 and Testis