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Histidine Triad Nucleotide-Binding Protein 1 (HINT1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS623190

Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor… More details

$850 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Histidine Triad Nucleotide-binding Protein 1 (HINT1), Polyclonal Antibody
Full Product Name
Histidine Triad Nucleotide-binding Protein 1 (HINT1, Adenosine 5'-monophosphoramidase, FLJ30414, FLJ32340, HINT, PKCI1, PKCI-1, PRKCNH1, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1)
Product Gene Name
Product Synonym Names
Anti -Histidine Triad Nucleotide-binding Protein 1 (HINT1, Adenosine 5'-monophosphoramidase, FLJ30414, FLJ32340, HINT, PKCI1, PKCI-1, PRKCNH1, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human HINT1. Species Crossreactivity: Mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
13,802 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (130494875..130501034, complement). Location: 5q31.2
OMIM
601314
3D Structure
ModBase 3D Structure for P49773
Immunogen
Synthetic peptide corresponding to 17aa from near the center of the human HINT1.
Positive Control
Jurkat Cell Lysate
Applications Tested/Suitable for anti-HINT1 antibody
Western Blot (WB)
Application Notes for anti-HINT1 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1-2ug/ml

Western Blot (WB) of anti-HINT1 antibody

Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-HINT1 Polyclonal Antibody – Cat# MBS623190

Other Notes

Small volumes of anti-HINT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HINT1 purchase

MBS623190 is an antibody targeting HINT1. Also known as Histidine Triad Nucleotide-binding Protein 1 (HINT1, Adenosine 5'-monophosphoramidase, FLJ30414, FLJ32340, HINT, PKCI1, PKCI-1, PRKCNH1, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HINT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histidine triad nucleotide-binding protein 1
NCBI Official Synonym Full Names
histidine triad nucleotide binding protein 1
NCBI Official Symbol
HINT1
NCBI Official Synonym Symbols
HINT; PKCI-1; PRKCNH1; FLJ30414; FLJ32340
UniProt Protein Name
Histidine triad nucleotide-binding protein 1
UniProt Synonym Protein Names
Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1
Protein Family
UniProt Gene Name
HINT1
UniProt Synonym Gene Names
HINT; PKCI1; PRKCNH1
UniProt Entry Name
HINT1_HUMAN
NCBI Protein Information
histidine triad nucleotide-binding protein 1; OTTHUMP00000159244; protein kinase C inhibitor 1; adenosine 5'-monophosphoramidase; protein kinase C-interacting protein 1

NCBI Summary for HINT1

The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012]
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UniProt Comments for HINT1

HINT1: Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha- acetyl lysine methyl ester and AMP-NH2. Belongs to the HINT family.

Protein type: EC 3.-.-.-; Cytoskeletal; Hydrolase

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoskeleton; histone deacetylase complex; cytoplasm; plasma membrane; nucleus

Molecular Function: protein kinase C binding; hydrolase activity; nucleotide binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; purine ribonucleotide catabolic process; signal transduction

Disease: Neuromyotonia And Axonal Neuropathy, Autosomal Recessive
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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