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MMP15 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS623391

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as… More details

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Product Overview

Online Datasheet in PDF
Product Name
MMP15, Polyclonal Antibody
Full Product Name
MMP15, NT (Matrix Metalloproteinase-15, MMP-15, Membrane-type Matrix Metalloproteinase 2, MT-MMP 2, MTMMP2, Membrane-type-2 Matrix Metalloproteinase, MT2-MMP, MT2MMP, SMCP-2)
Product Gene Name
Product Synonym Names
Anti -MMP15, NT (Matrix Metalloproteinase-15, MMP-15, Membrane-type Matrix Metalloproteinase 2, MT-MMP 2, MTMMP2, Membrane-type-2 Matrix Metalloproteinase, MT2-MMP, MT2MMP, SMCP-2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human MMP15. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
75,807 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (58059282..58080805). Location: 16q13
OMIM
602261
3D Structure
ModBase 3D Structure for P51511
Immunogen
Synthetic peptide selected from the N-terminal region of human MMP15 (KLH).
Applications Tested/Suitable for anti-MMP15 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-MMP15 antibody
Suitable for use in ELISA, Western Blot, Flow Cytometry, and Immunohistochemistry.
Dilution: Western Blot: 1:100-1:500
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-1:50

Western Blot (WB) of anti-MMP15 antibody

The anti-MMP15 N-term Antibody is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.
Rabbit Anti-MMP15 Polyclonal Antibody – Cat# MBS623391

Immunohistochemistry (IHC) of anti-MMP15 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma with MMP15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-MMP15 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS623391

Flow Cytometry (FC/FACS) of anti-MMP15 antibody

Flow cytometric analysis of WiDr cells using MMP15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-MMP15 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS623391

Other Notes

Small volumes of anti-MMP15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP15 purchase

MBS623391 is an antibody targeting MMP15. Also known as MMP15, NT (Matrix Metalloproteinase-15, MMP-15, Membrane-type Matrix Metalloproteinase 2, MT-MMP 2, MTMMP2, Membrane-type-2 Matrix Metalloproteinase, MT2-MMP, MT2MMP, SMCP-2). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP15. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
matrix metalloproteinase-15 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 15 (membrane-inserted)
NCBI Official Symbol
MMP15
NCBI Official Synonym Symbols
MTMMP2; SMCP-2; MT2-MMP
UniProt Protein Name
Matrix metalloproteinase-15
UniProt Synonym Protein Names
Membrane-type matrix metalloproteinase 2; MT-MMP 2; MTMMP2; Membrane-type-2 matrix metalloproteinase; MT2-MMP; MT2MMP; SMCP-2
UniProt Gene Name
MMP15
UniProt Entry Name
MMP15_HUMAN
NCBI Protein Information
matrix metalloproteinase-15; MMP-15; MT2MMP; MT-MMP 2; OTTHUMP00000164671; membrane-type matrix metalloproteinase 2; membrane-type-2 matrix metalloproteinase; matrix metalloproteinase 15 (membrane-inserted)

NCBI Summary for MMP15

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. [provided by RefSeq]
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UniProt Comments for MMP15

MMP15: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. Belongs to the peptidase M10A family.

Protein type: Membrane protein, integral; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular matrix; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; protein modification process; proteolysis; response to estradiol stimulus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MMP15 antibody

 Reference Product  PubMed Publications
 LRP1 antibody  >1 publications with MMP15 and LRP1

Pathways associated with anti-MMP15 antibody

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