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EARS2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS623737

Glutamyl-tRNA synthetase (GluRS or EARS2) a class I aminoacyl-tRNA synthetase (aaRS), is primarily responsible for the glutamylation of tRNAGlu. It is part of the "minimal set" of seventeen aaRSs found in every living organism and its presence is essential… More details

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Product Overview

Online Datasheet in PDF
Product Name
EARS2, Polyclonal Antibody
Full Product Name
EARS2, CT (Probable Glutamyl-tRNA Synthetase, Mitochondrial, Glutamate-tRNA Ligase, GluRS, KIAA1970)
Product Gene Name
Product Synonym Names
Anti -EARS2, CT (Probable Glutamyl-tRNA Synthetase, Mitochondrial, Glutamate-tRNA Ligase, GluRS, KIAA1970)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human EARS2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
59,959 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (23533334..23568696, complement). Location: 16p12.2
3D Structure
ModBase 3D Structure for Q86YH3
Immunogen
Synthetic peptide selected from the C-terminal region of human EARS2 (KLH).
Applications Tested/Suitable for anti-EARS2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EARS2 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50

Western Blot (WB) of anti-EARS2 antibody

Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane).EARS2 (arrow) was detected using the purified Pab.
Rabbit Anti-EARS2 Polyclonal Antibody – Cat# MBS623737

Immunohistochemistry (IHC) of anti-EARS2 antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-EARS2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS623737

Other Notes

Small volumes of anti-EARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EARS2 purchase

MBS623737 is an antibody targeting EARS2. Also known as EARS2, CT (Probable Glutamyl-tRNA Synthetase, Mitochondrial, Glutamate-tRNA Ligase, GluRS, KIAA1970). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EARS2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
EARS2 protein
NCBI Official Synonym Full Names
glutamyl-tRNA synthetase 2, mitochondrial (putative)
NCBI Official Symbol
EARS2
NCBI Official Synonym Symbols
MSE1; KIAA1970
UniProt Protein Name
EARS2 protein
UniProt Gene Name
EARS2
UniProt Entry Name
Q86YH3_HUMAN
NCBI Protein Information
probable glutamyl-tRNA synthetase, mitochondrial; gluRS; glutamate--tRNA ligase; glutamate tRNA ligase 2, mitochondrial

NCBI Summary for EARS2

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class-I aminoacyl-tRNA synthetase family and catalyzes the attachment of glutamate to tRNA(Glu). Alternative splicing results in multiple transcript variants. [provided by RefSeq]
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UniProt Comments for EARS2

EARS2: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: Translation; Mitochondrial; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 6.1.1.17; Ligase

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: glutamate-tRNA(Gln) ligase activity; glutamate-tRNA ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; glutamyl-tRNA aminoacylation; gene expression

Disease: Combined Oxidative Phosphorylation Deficiency 12
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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