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Myogef (MYH10) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS623916

Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. It Also activates RHOG and does not activate RAC1, or to a much lower extent than RHOA and RHOG. Its part of a functional unit, involving… More details

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Product Overview

Online Datasheet in PDF
Product Name
MyoGEF (MYH10), Polyclonal Antibody
Full Product Name
MyoGEF, NT (Myosin-interacting Guanine Nucleotide Exchange Factor, FLJ10665, MGC126315, MGC126353, MGC126354, Pleckstrin Homology Domain-containing Family G Member 6, PH Domain-containing Family G Member 6, PLEKHG6)
Product Gene Name
anti-MYH10 antibody
Product Synonym Names
Anti -MyoGEF, NT (Myosin-interacting Guanine Nucleotide Exchange Factor, FLJ10665, MGC126315, MGC126353, MGC126354, Pleckstrin Homology Domain-containing Family G Member 6, PH Domain-containing Family G Member 6, PLEKHG6)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MyoGEF.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
228,999 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (8377523..8534094, complement). Location: 17p13
OMIM
160776
3D Structure
ModBase 3D Structure for P35580
Immunogen
Synthetic peptide selected from the N-terminal region of human MyoGEF (KLH).

Product Categories/Family for anti-MYH10 antibody

Antibodies; Abs to Myosin
Applications Tested/Suitable for anti-MYH10 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-MYH10 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500

Western Blot (WB) of anti-MYH10 antibody

Western blot analysis of MyoGEF Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). MyoGEF (arrow) was detected using the purified Pab.
Rabbit Anti-MYH10 Polyclonal Antibody – Cat# MBS623916

Immunohistochemistry (IHC) of anti-MYH10 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-MYH10 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS623916

Flow Cytometry (FC/FACS) of anti-MYH10 antibody

Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-MYH10 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS623916

Other Notes

Small volumes of anti-MYH10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYH10 purchase

MBS623916 is an antibody targeting MYH10. Also known as MyoGEF, NT (Myosin-interacting Guanine Nucleotide Exchange Factor, FLJ10665, MGC126315, MGC126353, MGC126354, Pleckstrin Homology Domain-containing Family G Member 6, PH Domain-containing Family G Member 6, PLEKHG6). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MYH10. It may not necessarily be applicable to this product.

NCBI GI #
215274129
NCBI GeneID
4628
NCBI Accession #
P35580.3
UniProt Primary Accession #
P35580
UniProt Secondary Accession #
Q12989; Q149N3; Q149N4; Q16087; Q4LE45; Q6PK16; F8VTL3
UniProt Related Accession #
P35580
NCBI Official Full Name
Myosin-10
NCBI Official Synonym Full Names
myosin, heavy chain 10, non-muscle
NCBI Official Symbol
MYH10
NCBI Official Synonym Symbols
NMMHCB; NMMHC-IIB
UniProt Protein Name
Myosin-10
UniProt Synonym Protein Names
Cellular myosin heavy chain, type B; Myosin heavy chain 10; Myosin heavy chain, non-muscle IIb; Non-muscle myosin heavy chain B; NMMHC-B; Non-muscle myosin heavy chain IIb
Protein Family
Myosin
UniProt Gene Name
MYH10
UniProt Synonym Gene Names
NMMHC-B; NMMHC II-b; NMMHC-IIB
UniProt Entry Name
MYH10_HUMAN
NCBI Protein Information
myosin-10; nonmuscle myosin heavy chain IIB; nonmuscle myosin II heavy chain-B; cellular myosin heavy chain, type B; myosin heavy chain, nonmuscle type B; myosin, heavy polypeptide 10, non-muscle

NCBI Summary for MYH10

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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UniProt Comments for MYH10

MYH10: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with PLEKHG6. Interacts with ECM29. Interacts with KIF26B. Interacts with LARP6. Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: mitochondrion; myosin II complex; dendritic spine; actomyosin; cell cortex; actin cytoskeleton; growth cone; cell soma; axon; lamellipodium; cytoplasm; plasma membrane; stress fiber; spindle; midbody; myosin complex; nucleus; neuromuscular junction; cleavage furrow

Molecular Function: calmodulin binding; actin filament binding; microfilament motor activity; protein binding; actin binding; actin-dependent ATPase activity; ADP binding; ATP binding

Biological Process: adult heart development; axon guidance; exocytosis; metabolic process; in utero embryonic development; actin filament-based movement; fourth ventricle development; ventricular cardiac muscle cell development; actomyosin structure organization and biogenesis; neuron migration; cerebellar Purkinje cell layer development; cardiac myofibril assembly; third ventricle development; substrate-bound cell migration, cell extension; regulation of cell shape; cell proliferation; cytokinesis after mitosis; plasma membrane repair; retina development in camera-type eye; ephrin receptor signaling pathway; cell adhesion; neuromuscular process controlling balance; nuclear migration; lateral ventricle development
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Research Articles on MYH10

1. The myosin-IIB is unpolarized in cells on soft matrix in 2D and also within soft 3D collagen, with rearward polarization of MIIB emerging only as cells migrate from soft to stiff matrix.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MYH10 antibody

 Reference Product  PubMed Publications
 MYH9 antibody  >9 publications with MYH10 and MYH9
 MYL9 antibody  >2 publications with MYH10 and MYL9
 MYL12A antibody  >1 publications with MYH10 and MYL12A
 MYH11 antibody  >1 publications with MYH10 and MYH11
 MYL6 antibody  >1 publications with MYH10 and MYL6

Pathways associated with anti-MYH10 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Sema4D In Semaphorin Signaling Pathway antibodies  Sema4D In Semaphorin Signaling Pathway Diagram
 Sema4D Induced Cell Migration And Growth-cone Collapse Pathway antibodies  Sema4D Induced Cell Migration And Growth-cone Collapse Pathway Diagram
 Semaphorin Interactions Pathway antibodies  Semaphorin Interactions Pathway Diagram

Diseases associated with anti-MYH10 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with MYH10 and Neoplasms
 Nervous System Diseases Antibodies  >2 publications with MYH10 and Nervous System Diseases
 Heart Diseases Antibodies  >1 publications with MYH10 and Heart Diseases
 Hypertension Antibodies  >1 publications with MYH10 and Hypertension
 Disease Models, Animal Antibodies  >1 publications with MYH10 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >1 publications with MYH10 and Cardiovascular Diseases
 Kidney Diseases Antibodies  >1 publications with MYH10 and Kidney Diseases
 Glomerulosclerosis, Focal Segmental Antibodies  >1 publications with MYH10 and Glomerulosclerosis, Focal Segmental

Organs/Tissues associated with anti-MYH10 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >7 publications with MYH10 and Muscle
 Heart Antibodies  >4 publications with MYH10 and Heart
 Brain Antibodies  >4 publications with MYH10 and Brain
 Blood Antibodies  >2 publications with MYH10 and Blood
 Ear Antibodies  >1 publications with MYH10 and Ear
 Intestine Antibodies  >1 publications with MYH10 and Intestine
 Prostate Antibodies  >1 publications with MYH10 and Prostate
 Kidney Antibodies  >1 publications with MYH10 and Kidney
 Testis Antibodies  >1 publications with MYH10 and Testis
 Eye Antibodies  >1 publications with MYH10 and Eye
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