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ZW10 Interactor 1 (ZWINT) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS624147

ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores… More details

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Product Overview

Online Datasheet in PDF
Product Name
ZW10 Interactor 1 (Zwint), Polyclonal Antibody
Full Product Name
ZW10 Interactor 1 (ZW10-interacting Protein 1, ZWINT 1, ZWINT1, ZWINT-1, ZW10 Interactor, ZWINT, Human ZW10 Interacting Protein 1, HZwint 1, HZwint-1, KNTC2AP, MGC117174)
Product Gene Name
anti-Zwint antibody
Product Synonym Names
Anti -ZW10 Interactor 1 (ZW10-interacting Protein 1, ZWINT 1, ZWINT1, ZWINT-1, ZW10 Interactor, ZWINT, Human ZW10 Interacting Protein 1, HZwint 1, HZwint-1, KNTC2AP, MGC117174)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ZWINT. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
30,142 Da
Chromosome Location
Chromosome: 20; NC_005119.3 (19258272..19274206, complement). Location: chromosome: 20
3D Structure
ModBase 3D Structure for Q8VIL3
Immunogen
KLH-conjugated synthetic peptide between amino acids 66-96 in the center region of human ZWINT

Product Categories/Family for anti-Zwint antibody

Antibodies; Abs to Mitosis Proteins
Applications Tested/Suitable for anti-Zwint antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-Zwint antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Western Blot: 1:50-1:100

Western Blot (WB) of anti-Zwint antibody

Western blot analysis of ZWINT antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). ZWINT (arrow) was detected using the purified Pab.
Rabbit Anti-ZWINT Polyclonal Antibody – Cat# MBS624147

Immunohistochemistry (IHC) of anti-Zwint antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ZWINT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ZWINT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS624147

Flow Cytometry (FC/FACS) of anti-Zwint antibody

Flow cytometric analysis of widr cells using ZWINT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ZWINT Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS624147

Western Blot (WB) of anti-Zwint antibody

Western blot analysis of ZWINT (arrow) using rabbit polyclonal ZWINT Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ZWINT gene.
Rabbit Anti-ZWINT Polyclonal Antibody, Western Blot (WB) – Cat# MBS624147

Other Notes

Small volumes of anti-Zwint antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Zwint purchase

MBS624147 is an antibody targeting Zwint. Also known as ZW10 Interactor 1 (ZW10-interacting Protein 1, ZWINT 1, ZWINT1, ZWINT-1, ZW10 Interactor, ZWINT, Human ZW10 Interacting Protein 1, HZwint 1, HZwint-1, KNTC2AP, MGC117174). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Zwint. It may not necessarily be applicable to this product.

NCBI GI #
22129759
NCBI GeneID
257644
NCBI Accession #
NP_671479.1
UniProt Primary Accession #
Q8VIL3
UniProt Secondary Accession #
Q546Y5
UniProt Related Accession #
Q8VIL3
GenBank Nucleotide #
NM_147138.2
NCBI Official Full Name
ZW10 interactor
NCBI Official Synonym Full Names
ZW10 interacting kinetochore protein
NCBI Official Symbol
Zwint
NCBI Official Synonym Symbols
Sip30; Zwint-1
UniProt Protein Name
ZW10 interactor
UniProt Synonym Protein Names
SNAP25-interacting protein 30; Scoilin; ZW10-interacting protein 1
Protein Family
ZW10 interactor
UniProt Gene Name
Zwint
UniProt Synonym Gene Names
Sip30; Zwint-1
UniProt Entry Name
ZWINT_RAT
NCBI Protein Information
ZW10 interactor; scoilin; neuroprotective protein 8; ZW10 interacting protein-1; ZW10-interacting protein 1; SNAP25 interacting protein 30; SNAP25-interacting protein 30; ZW10 interactor, kinetochore protein

NCBI Summary for Zwint

directly interacts with SNAP25; may be involved in exocytosis of synaptic vesicles in neurotransmission [RGD, Feb 2006]

UniProt Comments for Zwint

ZWINT: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.

Protein type: Cell cycle regulation

Cellular Component: kinetochore; neuron projection; intracellular membrane-bound organelle; dendrite; cytoplasm; nucleus

Molecular Function: protein binding; protein N-terminus binding

Biological Process: mitotic sister chromatid segregation; cell division; mitotic cell cycle checkpoint; establishment of cellular localization
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Research Articles on Zwint

1. there is an ERK-responsive region in the rat sip30 promoter. Activation of ERK promotes the recruitment of the transcription factor cyclic AMP-response element-binding protein to the sip30 gene promoter

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Zwint antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 Resolution Of Sister Chromatid Cohesion Pathway antibodies  Resolution Of Sister Chromatid Cohesion Pathway Diagram
 Separation Of Sister Chromatids Pathway antibodies  Separation Of Sister Chromatids Pathway Diagram

Diseases associated with anti-Zwint antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with Zwint and Neoplasms
 Carcinoma Antibodies  >2 publications with Zwint and Carcinoma
 Congenital Abnormalities Antibodies  >2 publications with Zwint and Congenital Abnormalities
 Breast Neoplasms Antibodies  >2 publications with Zwint and Breast Neoplasms
 Lung Neoplasms Antibodies  >1 publications with Zwint and Lung Neoplasms
 Prostatic Diseases Antibodies  >1 publications with Zwint and Prostatic Diseases
 Urinary Bladder Neoplasms Antibodies  >1 publications with Zwint and Urinary Bladder Neoplasms
 Cell Transformation, Neoplastic Antibodies  >1 publications with Zwint and Cell Transformation, Neoplastic
 Abnormalities, Drug-Induced Antibodies  >1 publications with Zwint and Abnormalities, Drug-Induced
 Fibrosis Antibodies  >1 publications with Zwint and Fibrosis

Organs/Tissues associated with anti-Zwint antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >9 publications with Zwint and Nerve
 Brain Antibodies  >5 publications with Zwint and Brain
 Testis Antibodies  >2 publications with Zwint and Testis
 Muscle Antibodies  >1 publications with Zwint and Muscle
 Prostate Antibodies  >1 publications with Zwint and Prostate
 Embryonic Tissue Antibodies  >1 publications with Zwint and Embryonic Tissue
 Kidney Antibodies  >1 publications with Zwint and Kidney
 Lung Antibodies  >1 publications with Zwint and Lung
 Inner Ear Antibodies  >1 publications with Zwint and Inner Ear
 Eye Antibodies  >1 publications with Zwint and Eye
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