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Phospholipase A2 (sPLA2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6241499

MBS6241499 is an antibody targeting sPLA2. Also known as Phospholipase A2, Secretory (PLA2, sPLA2) (AP). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phospholipase A2 (sPLA2), Monoclonal Antibody
Full Product Name
Phospholipase A2, Secretory (PLA2, sPLA2) (AP)
Product Gene Name
anti-sPLA2 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
0.T.169
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human type II secretory PLA2 at ~14kD. Recognizes PLA2 in sperm, disc and synovial fluid.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
144
OMIM
172411
3D Structure
ModBase 3D Structure for P14555
Immunogen
HPLC-purified human sperm phospholipase A2 (PLA2)
Conjugate
AP

Product Categories/Family for anti-sPLA2 antibody

Antibodies; Enzymes; Phospholipase
Applications Tested/Suitable for anti-sPLA2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-sPLA2 antibody
ELISA: 1:300 detects 3-28ng PLA2
IHC: Detects PLA2 in colorectal resection specimens.
WB: 5-10ug/ml detects 10ng PLA2 under non-reducing conditions. Human synovial PLA2 was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS601861. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-sPLA2 antibody

Western Blot Analysis: 5-10ug/ml of this lot detected 10ng PLA2 under non-reducing conditions. Western Blot Analysis Human synovial PLA2 was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS601861 (5ug/ml). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PLA2.
Mouse Anti-sPLA2 Monoclonal Antibody – Cat# MBS6241499

Product References and Citations for anti-sPLA2 antibody

1. Edhemovic I., et al., Eur. J. Surg. Oncol., 27:545-548, 2001.

Other Notes

Small volumes of anti-sPLA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for sPLA2. It may not necessarily be applicable to this product.

NCBI GI #
129483
NCBI GeneID
5320
NCBI Accession #
P14555.2
UniProt Primary Accession #
P14555
UniProt Secondary Accession #
Q6DN24; Q6IBD9; Q9UCD2; A8K5I7
UniProt Related Accession #
P14555
GenBank Nucleotide #
NM_000300.2
NCBI Official Full Name
Phospholipase A2, membrane associated
NCBI Official Synonym Full Names
phospholipase A2, group IIA (platelets, synovial fluid)
NCBI Official Symbol
PLA2G2A
NCBI Official Synonym Symbols
MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
UniProt Protein Name
Phospholipase A2, membrane associated
UniProt Synonym Protein Names
GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A
Protein Family
Phospholipase A2 A2-actitoxin
UniProt Gene Name
PLA2G2A
UniProt Synonym Gene Names
PLA2B; PLA2L; RASF-A; NPS-PLA2
UniProt Entry Name
PA2GA_HUMAN
NCBI Protein Information
phospholipase A2, membrane associated; NPS-PLA2; GIIC sPLA2; group IIA phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A; non-pancreatic secretory phospholipase A2

NCBI Summary for sPLA2

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]
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UniProt Comments for sPLA2

PLA2G2A: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Vesicle; Endoplasmic reticulum; Phospholipase; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Lipid Metabolism - alpha-linolenic acid; Mitochondrial; Secreted; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 1p35

Cellular Component: extracellular space; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; extracellular region; secretory granule

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; phospholipid binding; calcium ion binding

Biological Process: defense response to Gram-positive bacterium; phosphatidic acid metabolic process; somatic stem cell maintenance; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process; positive regulation of inflammatory response; negative regulation of epithelial cell proliferation

Disease: Colorectal Cancer
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Research Articles on sPLA2

1. PLA2G2A gene polymorphisms may modify the risk of esophageal squamous cell carcinoma development

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-sPLA2 antibody

 Reference Product  PubMed Publications
 PTGS2 antibody  >7 publications with sPLA2 and PTGS2
 PLA2G7 antibody  >2 publications with sPLA2 and PLA2G7
 PTGS1 antibody  >2 publications with sPLA2 and PTGS1

Pathways associated with anti-sPLA2 antibody

 Products by Pathway  Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Acyl Chain Remodelling Of PE Pathway antibodies  Acyl Chain Remodelling Of PE Pathway Diagram
 Acyl Chain Remodelling Of PG Pathway antibodies  Acyl Chain Remodelling Of PG Pathway Diagram
 Acyl Chain Remodelling Of PI Pathway antibodies  Acyl Chain Remodelling Of PI Pathway Diagram
 Acyl Chain Remodelling Of PS Pathway antibodies  Acyl Chain Remodelling Of PS Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram

Diseases associated with anti-sPLA2 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >37 publications with sPLA2 and Inflammation
 Colorectal Neoplasms Antibodies  >25 publications with sPLA2 and Colorectal Neoplasms
 Colonic Neoplasms Antibodies  >19 publications with sPLA2 and Colonic Neoplasms
 Heart Diseases Antibodies  >8 publications with sPLA2 and Heart Diseases
 Atherosclerosis Antibodies  >8 publications with sPLA2 and Atherosclerosis
 Lung Neoplasms Antibodies  >6 publications with sPLA2 and Lung Neoplasms
 Necrosis Antibodies  >5 publications with sPLA2 and Necrosis
 Myocardial Ischemia Antibodies  >5 publications with sPLA2 and Myocardial Ischemia
 Liver Diseases Antibodies  >4 publications with sPLA2 and Liver Diseases
 Hyperplasia Antibodies  >4 publications with sPLA2 and Hyperplasia

Organs/Tissues associated with anti-sPLA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >38 publications with sPLA2 and Intestine
 Heart Antibodies  >11 publications with sPLA2 and Heart
 Lung Antibodies  >10 publications with sPLA2 and Lung
 Stomach Antibodies  >9 publications with sPLA2 and Stomach
 Liver Antibodies  >7 publications with sPLA2 and Liver
 Vascular Antibodies  >7 publications with sPLA2 and Vascular
 Prostate Antibodies  >5 publications with sPLA2 and Prostate
 Brain Antibodies  >3 publications with sPLA2 and Brain
 Uterus Antibodies  >3 publications with sPLA2 and Uterus
 Muscle Antibodies  >2 publications with sPLA2 and Muscle
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